[Histonet] double trouble

[Paula Pierce]

Incubate with one antibody and use DAB as the chromagen, then go back and incubate with the second antibody and use a colored chromagen such as AEC. 


Paula



----- Original Message ----
From: "Edwards, R.E.." <ree3 <@t> leicester.ac.uk>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, November 3, 2008 8:08:46 AM
Subject: [Histonet] double trouble


OK chaps, which in your opinion is the  best double labelling method when one  is  using primary antibodies raised in the  same  species, I am aware that Vector labs supply such a kit; many  thanks..

                                      Cheers
                                            Richard Edwards
                                                        University of Leicester

                                                                            U.K....
                              

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