[Histonet] (no subject)

[Patsy Ruegg]

I speed up the fixation and decal time by using a platform shaker for both,
it enhances the exchange, especially for decal, I would not skimp on
fixation before decal, the fixation will protect the tissue from decal and
processing, if you do not adequately fix, you can lose the proteins of
interest during processing, and that is lose completely so that no retrieval
process will work to bring them back.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Troutman,
Kenneth A
Sent: Friday, October 31, 2008 12:18 PM
To: Histonet
Subject: [Histonet] (no subject)

Try the following decal from Surgipath.  We used it with great success when
I was in another lab.  It is a 2 step decal procedure and it can
significantly cut down on the total time.
 
http://www.surgipath.com/region/us/product.aspx?p=77
 
Ashley Troutman BS, HT(ASCP)QIHC
Histopathology Laboratory
Department of Pathology
Vanderbilt University Medical Center
Nashville, TN
<http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/>
<http://www.mc.vanderbilt.edu/> <http://www.vanderbilthealth.com/main/>  
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson
Sent: Thursday, October 30, 2008 11:44 AM
To: histonet
Subject: [Histonet] bone marrow biopsies

We are currently using 10% formalin fixation on our bone marrow cores.  We
fix for 2 hours minimum prior to decal.  We are using Immunocal from Decal
Corp. for 2-4 hrs followed with processing overnight in VIP.  Cores are
still crunchy upon sectioning and we are doing surface decal for up to 30
min. Our paths want cores turned out within 24 hrs following procedure. Any
suggestions?

Thanks.
Cindi

<http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/>
<http://www.mc.vanderbilt.edu/> <http://www.vanderbilthealth.com/main/>  
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