[Histonet] p53
Sebree Linda A.
LSebree <@t> uwhealth.org
Thu May 22 15:12:12 CDT 2008
Hi Jean,
We use Biocare Medical's p53, clone DO-7. It works well for us at 1:50 on our VMS instruments but we do use a pretty long on-line HIER and an amplification step on 2 of our 3 instruments.
Take care,
Linda
Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Thursday, May 22, 2008 3:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] p53
Hi everyone,
I am wondering what clone of p53 labs are using. I currently use Dako's DO-7 clone but am also testing NeoMarker's rabbit monoclonal SP5. I've gotten very different results comparing the two and would like to know what the majority of labs are using.
Thanks!
Jean Taylor HT(ASCP)QIHC
Meriter Labs
Madison, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, May 22, 2008 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 54, Issue 35
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Today's Topics:
1. Factor 13a (Sebree Linda A.)
2. Histology Supervisor - IHC (Melissa Ribeiro)
3. RE: Hematoxylin shortage (Burton, Lynn)
4. PH of Toluidine blue and aldehyde fuscin staining (FU,DONGTAO)
5. osmium (Nuernberger, Barb)
6. Manual BVD Procedure (Breeden, Sara)
7. HIER to repair dried specimens? (crochieresteve <@t> aol.com)
8. Re: osmium (Philip Oshel)
9. Re: Hematoxylin shortage (Cynthia Robinson)
10. Microtome blades jammed in dispensing box (Orla Gallagher)
11. RE: Microtome blades jammed in dispensing box (Roberta Horner)
12. Re: HIER to repair dried specimens? (Bryan Hewlett)
13. Leica Peloris (Salcedo, Rudy)
14. RE: Richard Allan Hematoxylin problems (Mary Abosso)
15. now hiring (godsgalnow <@t> aol.com)
16. Osmium (Paula Pierce)
17. Re: HIER to repair dried specimens? (crochieresteve <@t> aol.com)
18. Microtome blades jammed in dispenser box (Paula Pierce)
19. RE: Leica Peloris (Mike Pence)
20. RE: Hematoxylin shortage (Terri Brown)
----------------------------------------------------------------------
Message: 1
Date: Thu, 22 May 2008 09:10:01 -0500
From: "Sebree Linda A." <LSebree <@t> uwhealth.org>
Subject: [Histonet] Factor 13a
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<D7336540A20FB0479306D82CE05B8086011F5EFE <@t> uwhis-xchng4.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Good morning everyone,
My latest lot of Factor 13a, from a reputable vendor, has "pooped out"
It tests out fine at their facility but is much weaker on my Benchmark,
XT and NexES, with DAB detection, than the previous lot and is basically
negative with Alk. Phos. Red detection.
So my question is, which F13a are people using reliably on Ventana
instruments?
Thanks for the help,
Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
------------------------------
Message: 2
Date: Thu, 22 May 2008 10:24:22 -0400
From: "Melissa Ribeiro" <mribeiro <@t> brinegroup.com>
Subject: [Histonet] Histology Supervisor - IHC
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0D88BB18E51FE3449EB2D2F505936A7A4ADB9C <@t> brinedc.brine.local>
Content-Type: text/plain; charset="US-ASCII"
Our firm has been retained to identify a candidate with a strong IHC
background for a Histology Supervisor position in a Massachusetts
Teaching Hospital.
Any qualified and interested candidates, please feel free to send
resumes to mribeiro <@t> brinegroup.com or call (781) 272-3400
Melissa Ribeiro
Healthcare Division
Brine Group Staffing Solutions
20 Mall Road, Suite 225
Burlington, MA 01803
mribeiro <@t> brinegroup.com
Ph. (781) 272-3400 ext. 228
Fax (781) 494-3401
Visit our newly redesigned web site at www.brinegroup.com
<blocked::http://www.brinegroup.com/> , the intelligent choice for
staffing solutions.
This electronic message contains information from Brine Group Staffing
Solutions that may be privileged and confidential. The information is
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the sender immediately.
------------------------------
Message: 3
Date: Thu, 22 May 2008 09:23:09 -0500
From: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>
Subject: RE: [Histonet] Hematoxylin shortage
To: "KELLY BOYD" <kdboydhisto <@t> yahoo.com>, "histonet"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B34ED7468185B44AB6CECF566B1960D01A68E2 <@t> IL084EX110.illinois.gov>
Content-Type: text/plain; charset="iso-8859-1"
I was recently at a meeting for ISH, and this has been reported on this venue previously, and all vendors and some speakers confirmed this to be true. They do say that the shortage should clear up in a few months. They also said that stock piling was not a good idea. Better to stretch your supply as much as possible.
Lynn Burton
Animal Disease Lab
Galesburg,Il
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of KELLY BOYD
Sent: Thu 5/22/2008 8:51 AM
To: histonet
Subject: [Histonet] Hematoxylin shortage
Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor?
Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
Tele (252)-830-6866
Cell (252)-943-9527
Fax (252)-830-0032
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 22 May 2008 10:36:46 -0400 (EDT)
From: "FU,DONGTAO" <fudo <@t> ufl.edu>
Subject: [Histonet] PH of Toluidine blue and aldehyde fuscin staining
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1056368044.242941211467006107.JavaMail.osg <@t> osgjas01.cns.ufl.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
Hi, all
we will do some special staining of T blue and aldehyde fuscin.
We will make some solutions by ourself. I notice some buffers need
to measure PH value. But they are dyes, so you can not use dip
paper. How do you measure them?
Thank you,
Ann
------------------------------
Message: 5
Date: Thu, 22 May 2008 10:36:58 -0400
From: "Nuernberger, Barb" <BNuern <@t> coj.net>
Subject: [Histonet] osmium
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <66529604C8644845BB24FF6F6235664101FCC33C <@t> EVS1.coj.net>
Content-Type: text/plain; charset="us-ascii"
I'm asking this on behalf of my histologist who has been asked by a
pathologist to modify a protocol that she has from the AFIP manual.
When doing an osmium fixation on formalin fixed tissue, is the 8 hour
fixing time a minimum or can it sit overnight before continuing?
Thank you for any help.
Barb Nuernberger
Chief Toxicologist
Medical Examiner's Office
2100 Jefferson St.
Jacksonville,FL 32206
(904)630-5470
BNuern <@t> coj.net
------------------------------
Message: 6
Date: Thu, 22 May 2008 08:45:56 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Manual BVD Procedure
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: "Taylor, Flint" <FTaylor <@t> nmda.nmsu.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B017E631D <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
I have a visiting veterinary pathologist from Mexico this week; he is in
need of a manual procedure for BVD. I run the Ventana system and am no
help to him - could someone kindly share their manual BVD protocol with
me? And perhaps a source for the antibody for same? My thanks!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 7
Date: Thu, 22 May 2008 10:49:01 -0400
From: crochieresteve <@t> aol.com
Subject: [Histonet] HIER to repair dried specimens?
To: histonet <@t> pathology.swmed.edu
Message-ID: <8CA8A321B9E08F5-6B4-1295 <@t> webmail-nf15.sim.aol.com>
Content-Type: text/plain; charset="us-ascii"
I have accidentally stumbled on an unexplainable "by product" of HIER in citrate buffer, pH6.0.
We had some specimens that had unfortunately air dried prior to fixation and processing and therefore looked terrible. The H&E was practically unreadable due to drying artifact. In an effort to see something worthwhile, IHC stains were ordered. The hematoxylin counterstain, following retrieval showed much better nuclear detail than the original H&E. Several additional slides were cut and stained with H&E following HIER. They were much better. This has been repeated on a few other specimens with similar results. Any ideas as to how or why this is working?
thanks
sc
------------------------------
Message: 8
Date: Thu, 22 May 2008 11:03:38 -0400
From: Philip Oshel <oshel1pe <@t> cmich.edu>
Subject: Re: [Histonet] osmium
To: "Nuernberger, Barb" <BNuern <@t> coj.net>
Cc: Histonet <@t> Pathology.swmed.edu
Message-ID: <f06240807c45b3b161383@[141.209.160.249]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Discounting regulations, generally osmium fixation can be done
overnight at 4 deg C (refrigerator temperature). Mind, OsO4 is slow
penetrating, and overnight might be required simply because of the
size of the tissue blocks.
Room temp ... mayyyyyybbbeee given the tissue requirements. But OsO4
makes tissues brittle and crunchy, so that usually isn't a good idea.
Phil
>I'm asking this on behalf of my histologist who has been asked by a
>pathologist to modify a protocol that she has from the AFIP manual.
>When doing an osmium fixation on formalin fixed tissue, is the 8 hour
>fixing time a minimum or can it sit overnight before continuing?
>
>Thank you for any help.
>Barb Nuernberger
>Chief Toxicologist
>
>
>Medical Examiner's Office
>2100 Jefferson St.
>Jacksonville,FL 32206
>(904)630-5470
>BNuern <@t> coj.net
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
------------------------------
Message: 9
Date: Thu, 22 May 2008 11:11:54 -0400
From: "Cynthia Robinson" <Robinsoc <@t> mercyhealth.com>
Subject: Re: [Histonet] Hematoxylin shortage
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>, "KELLY BOYD"
<kdboydhisto <@t> yahoo.com>, "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Message-ID: <483546EA.59BC.00AF.0 <@t> mercyhealth.com>
Content-Type: text/plain; charset=UTF-8
I use Thermo Shandon as a supplier for my hematoxylin and just got an
order placed on back order until the end of 2008.
Cindi Robinson HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes, SD 57049
>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 5/22/2008 9:00 AM >>>
Never pay attention to a vendor urging to stock up. It just may be a
gimmick to increase sales/commissions. There is nothing concrete so far
about that rumor.
René J.
KELLY BOYD <kdboydhisto <@t> yahoo.com> wrote:
Can someone please tell me what is up with all the talk about a
hematoxylin shortage? There is even a vendor telling their customers
they need to stock up. Is this fact or rumor?
Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
Tele (252)-830-6866
Cell (252)-943-9527
Fax (252)-830-0032
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 22 May 2008 16:19:51 +0100
From: "Orla Gallagher" <o.m.gallagher <@t> sheffield.ac.uk>
Subject: [Histonet] Microtome blades jammed in dispensing box
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <48359D26.20553.15909D5 <@t> localhost>
Content-Type: text/plain; charset=US-ASCII
Dear Histonetters,
We've been having a problem over the past year or 2 with our
microtome blades becoming jammed in the dispensing box after
only a few have been used and wondered if this has happened to
anyone else? Our refurbished building has a cooling system
without humidity; would this cause the blades to become jammed?
It's happened with Feather S35 & N35 blades and Accu Edge
blades from different suppliers and in more than one lab in the
building. Any advice on how to get the blades out would be much
appreciated!
Many thanks,
Orla
******************************
Ms. Orla Gallagher
Academic Unit of Bone Biology
D Floor (DU20) Medical School
Henry Wellcome Laboratories for Medical Research
University of Sheffield
Beech Hill Road
Sheffield S10 2RX
www.sheffield.ac.uk
E-mail:o.m.gallagher <@t> sheffield.ac.uk
Tel: 0114 271 3783(lab)
0114 271 3337(office)
Fax: 0114 271 1711
------------------------------
Message: 11
Date: Thu, 22 May 2008 11:33:14 -0400
From: Roberta Horner <rjr6 <@t> psu.edu>
Subject: RE: [Histonet] Microtome blades jammed in dispensing box
To: Orla Gallagher <o.m.gallagher <@t> sheffield.ac.uk>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F12BB68C2BA80B4686A8EA261F80DD4513EA6F5B64 <@t> pdc.padlspsu.psu.edu>
Content-Type: text/plain; charset="us-ascii"
I have had this happen several times. I found that if the dispenser button is pulled back so the next blade is ready to dispense I don't have it happen. When it does happen I have taken the dispenser apart carefully and separate the blades into a box lid.
Roberta Horner HT/HTL
Penn State University
Animal Diagnostic Lab
------------------------------
Message: 12
Date: Thu, 22 May 2008 11:38:28 -0400
From: "Bryan Hewlett" <bhewlett <@t> cogeco.ca>
Subject: Re: [Histonet] HIER to repair dried specimens?
To: <histonet <@t> pathology.swmed.edu>, <crochieresteve <@t> aol.com>
Message-ID: <000501c8bc21$e4296580$6700a8c0 <@t> mainbox>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
It's called re-hydration!
Similar techniques are used for partial restoration of mummified tissue for
histological examination.
Bryan
\
----- Original Message -----
From: <crochieresteve <@t> aol.com>
To: <histonet <@t> pathology.swmed.edu>
Sent: Thursday, May 22, 2008 10:49 AM
Subject: [Histonet] HIER to repair dried specimens?
>I have accidentally stumbled on an unexplainable "by product" of HIER in
>citrate buffer, pH6.0.
> We had some specimens that had unfortunately air dried prior to fixation
> and processing and therefore looked terrible. The H&E was practically
> unreadable due to drying artifact. In an effort to see something
> worthwhile, IHC stains were ordered. The hematoxylin counterstain,
> following retrieval showed much better nuclear detail than the original
> H&E. Several additional slides were cut and stained with H&E following
> HIER. They were much better. This has been repeated on a few other
> specimens with similar resu
> lts. Any ideas as to how or why this is working?
> thanks
> sc
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 13
Date: Thu, 22 May 2008 10:39:53 -0500
From: "Salcedo, Rudy" <RSalcedo <@t> tmhs.org>
Subject: [Histonet] Leica Peloris
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0DE2321B1716814E9DF3B6753171E72201133632 <@t> EXCH9.tmh.tmhs>
Content-Type: text/plain; charset="iso-8859-1"
Histonet,
Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance.
Rudy Salcedo
Houston, Texas
NOTICE: Our e-mail address at Methodist has changed to tmhs.org
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Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008
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------------------------------
Message: 14
Date: Thu, 22 May 2008 09:43:18 -0600
From: "Mary Abosso" <mabosso <@t> unipathllc.com>
Subject: RE: [Histonet] Richard Allan Hematoxylin problems
To: "Shawn Leslie" <LeslieS <@t> vetmed.ufl.edu>, "Angela Bitting"
<akbitting <@t> geisinger.edu>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<43A451981FF6634795BE83B1B5494D6313647D <@t> exchange.unipathllc.corp>
Content-Type: text/plain; charset="iso-8859-1"
I was the QA Histotechnologist at Richard-Allan and the best way to handle any product issues is to call in to Tech-Rite technical support who can help figure out what the issue is. Additonally, they will file a complaint with the QA department who will thouroughly investigate the retain sample from that lot, all of the chemicals used in the batch. Richard-Allan (Thermo-Fisher Scientific) is committed to the highest standard of quality and needs the customer feed back to continuously improve.
Mary Abosso
Unipath LLC.
Denver, CO
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Shawn Leslie
Sent: Wed 5/21/2008 2:30 PM
To: Angela Bitting
Cc: HistoNet
Subject: Re: [Histonet] Richard Allan Hematoxylin problems
We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it....
Shawn Leslie HT (ASCP)
Scientific Research Manager
Anatomic Pathology
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4555
>>> "Angela Bitting" <akbitting <@t> geisinger.edu> 5/21/2008 3:18 PM >>>
We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!
>>> "Gayle Callis" <gayle.callis <@t> bresnan.net> 5/20/2008 4:00 PM >>>
We use xylene to remove coverslips and do this work inside a hood. After
coverslip is off and mounting media is gone with an extra xylene rinse, we
go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try
an minimize exposure to xylene is done. Unfortunately, we found the
substitute just didn't work for this job.
Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT
----- Original Message -----
From: "Patty Dunlop" <pdunlop720 <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping
> Hello Histonetters,
>
> I use Clear-Rite 3, a xylene substitute, and am having problems when I
> need
> to recoverslip. Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature. The coverslip
> really
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process. Does anyone have a method for removing coverslips with a xylene
> substitute? Should I use heat?
>
> Thanks,
> Patty
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
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------------------------------
Message: 15
Date: Thu, 22 May 2008 11:47:39 -0400
From: godsgalnow <@t> aol.com
Subject: [Histonet] now hiring
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CA8A3A4C2B73E8-D34-22E4 <@t> FWM-D26.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Hey---
I know that you all have seen my posting for our salary ranges here and it has caused much disagreement, sorry about that!? However, I am now looking for a histotechnologist (Florida licensed as a technologist, or eligible), HT(ASCP), with QIHC preferred.? Extensive knowledge of IHC is needed, prefer molecular testing background.? MP certification would be great.......
Excellent pay and bennies.......in sunny Tampa, Florida
And the best part is....you get to work with me (ha ha).
Roxanne
------------------------------
Message: 16
Date: Thu, 22 May 2008 08:48:51 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] Osmium
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <151803.29445.qm <@t> web50108.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Are the specimens to be used for electron microscopy or fat stains? How big are they?
Tell her to be very careful when using osmium. The fumes will fix your corneas.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway Ave.
Moore, OK 73160
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com
------------------------------
Message: 17
Date: Thu, 22 May 2008 11:51:48 -0400
From: crochieresteve <@t> aol.com
Subject: Re: [Histonet] HIER to repair dried specimens?
To: bhewlett <@t> cogeco.ca, histonet <@t> pathology.swmed.edu
Message-ID: <8CA8A3AE0814351-87C-14B7 <@t> webmail-nb18.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
I'm familiar with rehydrating mummy tissue from my years at AFIP. We used formol-glycol for several days prior to processing. I didn't think boiling slides in buffer would "repair" "cooked" tissue, but it seems to be so.
Thanks
s
-----Original Message-----
From: Bryan Hewlett <bhewlett <@t> cogeco.ca>
To: histonet <@t> pathology.swmed.edu; crochieresteve <@t> aol.com
Sent: Thu, 22 May 2008 11:38 am
Subject: Re: [Histonet] HIER to repair dried specimens?
It's called re-hydration!?
Similar techniques are used for partial restoration of mummified tissue for histological examination.?
?
Bryan?
\?
?
----- Original Message ----- From: <crochieresteve <@t> aol.com>?
To: <histonet <@t> pathology.swmed.edu>?
Sent: Thursday, May 22, 2008 10:49 AM?
Subject: [Histonet] HIER to repair dried specimens??
?
>I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0.?
> We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu?
> lts. Any ideas as to how or why this is working??
> thanks?
> sc?
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> Histonet <@t> lists.utsouthwestern.edu?
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> ?
------------------------------
Message: 18
Date: Thu, 22 May 2008 08:53:36 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] Microtome blades jammed in dispenser box
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <516735.64474.qm <@t> web50109.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I am having the same problem. The tab that grabs the hole in the blades to push them forward tends to wear more than in the past and does not grab after a few have been dispensed. I have taken a very fine pair of forceps and helped the blade along and when that no loner works because the blade does not move at all I took the box apart.
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway Ave.
Moore, OK 73160
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com
------------------------------
Message: 19
Date: Thu, 22 May 2008 11:18:45 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Leica Peloris
To: "Salcedo, Rudy" <RSalcedo <@t> tmhs.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A3849 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
I just purchased a Peloris about 3 months ago. Love it. I can process
all thru the day and it has made a huge impact on turn around times. We
cut fewer blocks thru out the day and I think it processes fatty
specimens better. I have a few small issues, but I have been very happy
with the processor.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Salcedo,
Rudy
Sent: Thursday, May 22, 2008 10:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Peloris
Histonet,
Any labs out there using the Leica Peloris Processor, if so please send
me some feed back on you experience with the Peloris. Thanks in advance.
Rudy Salcedo
Houston, Texas
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------------------------------
Message: 20
Date: Thu, 22 May 2008 12:21:46 -0400
From: "Terri Brown" <Terri.Brown <@t> Northside.com>
Subject: RE: [Histonet] Hematoxylin shortage
To: "Cynthia Robinson" <Robinsoc <@t> mercyhealth.com>, "histonet"
<Histonet <@t> lists.utsouthwestern.edu>, "KELLY BOYD"
<kdboydhisto <@t> yahoo.com>, "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Message-ID:
<8CEB6DA1A3F35743800669D4CFE21F7D03F8FFE0 <@t> NSMXMS04.northside.local>
Content-Type: text/plain; charset="iso-8859-1"
I heard that this was going to happen and ordered the powder so we could make our own. It is on backorder too. I ordered it back in April.
Terri Brown, HT (ASCP)
Northside Hospital-Atlanta, Ga.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson
Sent: Thursday, May 22, 2008 11:12 AM
To: histonet; KELLY BOYD; Rene J Buesa
Subject: Re: [Histonet] Hematoxylin shortage
I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008.
Cindi Robinson HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes, SD 57049
>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 5/22/2008 9:00 AM >>>
Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor.
René J.
KELLY BOYD <kdboydhisto <@t> yahoo.com> wrote:
Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor?
Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Harris Histology Services
2025 Eastgate Dr. Ste. F
Greenville, NC 27858
Tele (252)-830-6866
Cell (252)-943-9527
Fax (252)-830-0032
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End of Histonet Digest, Vol 54, Issue 35
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