[Histonet] Bone Marrow Staining

Rene J Buesa rjbuesa <@t> yahoo.com
Mon May 12 15:17:13 CDT 2008


The "washed out" appearance most likely has nothing to do with fixation. Check if you are heating the sections BEFORE they are completely drained off. That can cause that artifact.
  The only thing you have to be aware of when using NBF to fix BM specimens is to control the pH on the staining solutions (specially the Giemsa).
  René J.

Karla Arrington <freckles9660 <@t> yahoo.com> wrote:
  I currently use 10% NBF for fixing bone marrows (core and clot). Although lately
the sections appear "washed out". I previously used B-5 Fixative. Is there a better
fixative for fixing bone marrows so their cells are crisp and clear? 

Karla Arrington
freckles9660 <@t> yahoo.com



____________________________________________________________________________________
Be a better friend, newshound, and 
know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
Be a better friend, newshound, and know-it-all with Yahoo! Mobile.  Try it now.


More information about the Histonet mailing list