[Histonet] Why use Calcium Chloride in Trypsin Antigen Retrieval?

Thu May 8 14:14:59 CDT 2008

Does anyone know why CaCl2 is used in trypsin buffers for retrieval of 
antigen in paraformaldehyde-fixed, paraffin-embedded tissue sections?  Is 
it really necessary?  Can I just use a Trypsin-EDTA solution we already 
have in the lab?

Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725