[Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin)

Igor Nasonkin nasonkini <@t> mail.nih.gov
Tue May 6 18:24:14 CDT 2008


Hi everybody,

I am looking for a detailed protocol for PAS staining in liver sections
(paraffin-embedded). The 1st set of sections did not work, and we had no
+control since we have not done it. These are 4-6wk mouse liver sections
embedded in paraffin; our lab is experienced in IHC on paraffin sections so
these were done right. But PAS staining did not work. What could be main
reasons? Any positive control we could use? Thank you in advance,
Igor




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