[Histonet] Hematoxylin Shortage??

[kemlo]

We used to have 5 litre flasks of haematoxylin ripening in the window for
months; if you got taken short you had to artificially ripen using an
oxidising agent. If you had enough stock then the sun did the work.

I always thought "organic" haematoxylin stained better that the factory made
stuff but I do think it overoxidised at a slower rate and gave 'cleaner;
results.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: 29 March 2008 19:55
To: Gayle Callis; Emily Sours; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Shortage??

Hi All,

Yes, Gayle's memory is still going strong!  I remember
the boat rumor around 1976.  And yes, I too was making
Harris hematoxylin with mercuric oxide "red".  We
would let it ripen in the dark for a few weeks before
using it.  What a wonderful sheen it had.  I remember
a student was making it in the old Providence Hall
nursing school before we moved to our new lab.  The
student added the total amount of the mercuric oxide
and it shot straight up to the ceiling and permanently
stained it.   That was a heck of a lesson!

Regards,
Akemi

--- Gayle Callis <gayle.callis <@t> bresnan.net> wrote:

> Not the goat dying, but more like a boat sinking.
> 
> The last time there was a "rumored" hematoxylin
> shortage, back in the 70's, 
> it was some boat sinking with all the Campechium
> logs aboard.  I recall some 
> panic stockpiling of hematoxylin - the days when we
> made the stain ourselves 
> with mercuric oxide.  I think the sunken boat rumor
> was a figment of 
> someones vivid imagination.
> 
> For all the hematoxylin historians out there,
> correct me if I am wrong.
> 
> Gayle Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
> 
> 
> ----- Original Message ----- 
> From: "Emily Sours" <talulahgosh <@t> gmail.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Friday, March 28, 2008 7:25 AM
> Subject: Fwd: [Histonet] Hematoxylin Shortage??
> 
> 
> > Why would this happen?
> > Is this like that one goat dying at Santa Cruz?
> >
> > Emily
> > -- 
> > People aren't like chocolates. People are
> bastards. Bastards with
> > bastard coating and bastard filling.
> >
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu
> >
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
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Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100 
Seattle, WA 98103-8675
Work: (206) 374-9000
Cell: (425) 941-4287
E-Mail: akemiat3377 <@t> yahoo.com

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