[Histonet] Immuno on decaled toenail????

[Rene J Buesa]

First toenails do not contain calcium and the keratin they are made does not respond adequately to acid hydrolysis. You will need an alkaline hydrolysis (10% NaOH for at least 30 minutes after fixation before processing).
  After that the IHC is likely to be weak and you will need some twinkling.
  René J.

BSylinda <@t> aol.com wrote:
  Hello Histoland,
Is there any techs that have performed an immuno stains such as S100 on a 
toenail? Had any problems with staining after using a decal solution? I have 
stained twice and pathologist is not happy with staining pattern. This is 
the first nail that I've had to do a immunohistochemical stain on to rule out 
melanoma. Any other advice on softening toenail? Sorry for all of the 
questions, but I know this is a great place to start. Thanks in advance.

Sylinda Battle




**************Create a Home Theater Like the Pros. Watch the video on AOL 
Home. 
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
No Cost - Get a month of Blockbuster Total Access now. Sweet deal for Yahoo! users and friends.