[Histonet] Immuno on decaled toenail????
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Mar 29 09:57:21 CDT 2008
First toenails do not contain calcium and the keratin they are made does not respond adequately to acid hydrolysis. You will need an alkaline hydrolysis (10% NaOH for at least 30 minutes after fixation before processing).
After that the IHC is likely to be weak and you will need some twinkling.
BSylinda <@t> aol.com wrote:
Is there any techs that have performed an immuno stains such as S100 on a
toenail? Had any problems with staining after using a decal solution? I have
stained twice and pathologist is not happy with staining pattern. This is
the first nail that I've had to do a immunohistochemical stain on to rule out
melanoma. Any other advice on softening toenail? Sorry for all of the
questions, but I know this is a great place to start. Thanks in advance.
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