[Histonet] quenching GFP fluorescence

Jennifer MacDonald JMacDonald <@t> mtsac.edu
Fri Mar 28 12:58:00 CDT 2008

I'm not sure that this matters for this, but DAB is not a substrate, but a 
chromogen.  The actual substrate is hydrogen peroxide.

MKing <making <@t> ufl.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/28/2008 10:21 AM

histonet <@t> lists.utsouthwestern.edu

[Histonet] quenching GFP fluorescence

If you're using DAB as a peroxidase substrate for your IHC the GFP 
fluorescence will be eliminated.
Mike King
Message: 9
Date: Thu, 27 Mar 2008 15:01:26 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] quenching GFP fluorescence
To: histonet <@t> lists.utsouthwestern.edu
<OF7C33DB14.F32F4EAF-ON85257419.00680511-85257419.006882FA <@t> notes.health.state.ny.us>
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Hi Histonetters,
       How would you quench GFP fluorescence prior to IHC?
                                     Helen Johnson
(hej01 <@t> health.state.ny.us)

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