[Histonet] quenching GFP fluorescence

[Jennifer MacDonald]

I'm not sure that this matters for this, but DAB is not a substrate, but a 
chromogen.  The actual substrate is hydrogen peroxide.




MKing <making <@t> ufl.edu> 
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03/28/2008 10:21 AM

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[Histonet] quenching GFP fluorescence






Helen,
If you're using DAB as a peroxidase substrate for your IHC the GFP 
fluorescence will be eliminated.
Mike King
----------
Message: 9
Date: Thu, 27 Mar 2008 15:01:26 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] quenching GFP fluorescence
To: histonet <@t> lists.utsouthwestern.edu
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<OF7C33DB14.F32F4EAF-ON85257419.00680511-85257419.006882FA <@t> notes.health.state.ny.us>
 
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Hi Histonetters,
       How would you quench GFP fluorescence prior to IHC?
                                     Helen Johnson
(hej01 <@t> health.state.ny.us)


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