Why do you need to Re: [Histonet] quenching GFP fluorescence

[Gayle Callis]

Why do you need to get rid of GFP fluorescence if you are going to do IHC? 
IHC is based on using chromogen with enzyme immunohistochemistry and if you 
are not looking at your IHC protocol with a fluorescent microscope, but 
rather with  light microscopy, then GFP will not be seen - that would be 
more of a problem with doing immunofluorescent staining (IFA).

If you mean doing another immunofluorscent stain for different tissue 
component, or cell, then use a different color fluorophore i.e. red  than 
GFP, then you should not have GFP showing up, unless you switch to a GFP 
filter (you could have double fluorescence then).

Maybe I am not understanding what you want to do here.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 597175


----- Original Message ----- 
From: "Helen E Johnson" <hej01 <@t> health.state.ny.us>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, March 27, 2008 1:01 PM
Subject: [Histonet] quenching GFP fluorescence


>
> Hi Histonetters,
>      How would you quench GFP fluorescence prior to IHC?
>                                    Helen Johnson
> (hej01 <@t> health.state.ny.us)
>
>
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