[Histonet] Re: Histonet Digest, Vol 52, Issue 40

[William Gunn]

>
> We just X-ray the specimens, which avoids the risk of breaking/bending and
> it's easy to do all the samples at once.
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 27 Mar 2008 07:57:49 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Decal procedures-assessing endpoints
> To: Greg Dobbin <gvdobbin <@t> ihis.org>, Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <448114.89650.qm <@t> web65708.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> The "softness" of the tissue fragment, how it bends or compresses, will
> give you the most practical way of determining the decal end point.
>  Be careful with the bending to prevent breaking a piece not decalcified
> yet.
>  René J.
>
> Greg Dobbin <gvdobbin <@t> ihis.org> wrote:
>  Hello Colleagues,
> Can some of you share with me, your guidelines for determining
> appropriate submersion times for sections of bone or other calcified
> bits in RDO (Trade name) post-fixation but prior to processing
> (obviously). I guess it is a subjective call by necessity!?
> Thanks,
> Greg
>