[Histonet] mounting and staining celloidin-embedded whole brainsections

[patsy ruegg]

Jim,
I used to work with celloidin 30 years ago and it was a pain.  As I recall
we used alcohol and a fine spatula and fine brush, as the section came off
the block we dropped alcohol on it and painted it onto the surface of the
large steel or tungsten blade, scooped it up onto the spatula keeping it wet
with the alcohol, then we would slide it off the spatula on to the glass
microscope slide.  I think we dryed it on a heat plate flat from there
(60-70dc) but my memory is not so clear on that.
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of jstaruk
Sent: Thursday, March 27, 2008 9:06 AM
To: 'Histonet'
Subject: [Histonet] mounting and staining celloidin-embedded whole
brainsections

All,

 

I have 160 sections of celloidin-embedded Human brain sections cut at 50um
which now have to be mounted on glass and stained.  Is there anyone out
there who's done this before?  My main concern is how to mount the sections
to the glass and also staining methods (before or after mounting?).

 

Thank you

 

PS, for all of those who requested an AFB control block from me last week,
they were mailed out yesterday.

 

Jim

 

_____________________

     Jim Staruk

Mass Histology Service

www.masshistology.com


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