[Histonet] collagen coating

[Hazel Stevens]

Hi All,

 

I have received excellent responses from Histonet in the past so thought I
would try this out on you all. 

 

I am coating tissue culture plates with collagen and trying to demonstrate
complete coverage. Other than an antibody to collagen I , is there a simple
stain I could apply that would demonstrate coverage without dissolving the
collagen. Collagen seems to dissolve in methanol which seems to be a
component of some of the basic protein stains. Should I fix before staining?


Thanks for your help. Much appreciated.

Hazel 

 

Hazel Stevens, 

Georgia Tech,

Atlanta, Georgia 30332-0405 U.S.A.