[Histonet] collagen coating
hstevens3 <@t> mail.gatech.edu
Wed Mar 26 09:07:13 CDT 2008
I have received excellent responses from Histonet in the past so thought I
would try this out on you all.
I am coating tissue culture plates with collagen and trying to demonstrate
complete coverage. Other than an antibody to collagen I , is there a simple
stain I could apply that would demonstrate coverage without dissolving the
collagen. Collagen seems to dissolve in methanol which seems to be a
component of some of the basic protein stains. Should I fix before staining?
Thanks for your help. Much appreciated.
Atlanta, Georgia 30332-0405 U.S.A.
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