[Histonet] embedding problem small zebrafish

[Dickey, Coral]

Barbara,

Check out a paper in ScienceDirect available online at www.sciencedirect.com Entitled: High-throughput zebrafish histology by Sabaliauskas et. Al.

Good Luck!

Coral Dickey
Histology Specialist I

Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City,  Missouri  64110
Phone:  816-926-4305
e-mail:  cad <@t> stowers-institute.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea Hooper
Sent: Tuesday, March 25, 2008 10:16 AM
To: Barbara.Verstraeten <@t> UGent.be
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] embedding problem small zebrafish


Have you checked out the histological methods section of "The Zebrafish Book" by Monte Westerfield, on zfin.org available online here: http://zfin.org/zf_info/zfbook/cont.html#cont8
I don't think it has paraffin embedding protocols but perhaps there are some other hints will help? In any case, it's an amazing resource.

I am also wondering if it might help to embed in agarose before paraffin embedding - especially those 44 hour old critters who are tiny tiny tiny. Unfortunately most of my experience in that regard lies with frozen sectioning so I don't have too much to add.


----- Original Message -----
From: Barbara Verstraeten <barbara.verstraeten <@t> ugent.be>
Date: Tuesday, March 25, 2008 3:50 am
Subject: [Histonet] embedding problem small zebrafish

> Hello,
>
> I work on zebrafish from 44 hours to 6 days old (so they are very
> small; max. 0.5 cm). I would like to perform immostainings on them
> (such as E-cadherin staining). I've embedded my species in technovit
> 9100 but the cutting
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem
> is
> that my tissue is of very bad quality!! When I have 50 sections
> only 1
> is usefull.
> This is not very good!
>
> Can someone give me any suggestions on how to embed them so that they
> are more easy to section and that I can still stain them with
> different antibodies?
>
> Thank you all in advance!
>
> Kind regards,
>
> Barbara Verstraeten
> VMDB
> University of Ghent
> Belgium
>
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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>


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