[Histonet] ERCC1 IHC

[Min-Han Tan]

Dear fellow Histonetters,

I am planning to start immunostaining FFPE lung tissue for ERCC1.

The cited antibody in the relevant New England Journal of
Medicine<http://content.nejm.org/cgi/content/abstract/355/10/983>paper
ERCC1 Ab-2 (Clone 8F1), Neomarkers Cat. #MS-671-R7 (7.0ml)
did not work well for us in the prediluted form, and I understand the
prediluted form has been withdrawn by the manufacturer as well.

I would like to ask as to which antibody all of you are using for ERCC1
staining (lung cancer tissue for platinum sensitivity). I understand some
clinical laboratories in the United States are using it on a routine basis.
I am thinking of obtaining the Abcam one (with the same clone 8F1, approved
for IHC - it might even be the same antibody as the Neomarkers one!)

Thank you!

-- 
/min-han