[Histonet] Reprocessing tissue
jnocito <@t> satx.rr.com
Sun Mar 23 19:45:48 CDT 2008
ThermoFisher Scientific and Ventana Medical Systems have systems that you
can barcode the paperwork, which then makes bar-coded blocks which then
makes bar-coded slides. In each step, the barcodes are read to ensure that
the correct specimen is being processed. If there is a mis-match, an alarm
beeps alerting the user.
For reprocessing tissue, we just melt the blocks down, place the tissue back
in the block and put the blocks in formalin to be processed with new cases.
Whatever area did not process the first time will take up the formalin, and
graded alcohols. When the tissues reach xylene, the paraffin is dissolved
and everything get infiltrated. The areas that have been processed will
repel the formalin and alcohols until they are immersed into xylene. I find
this method is a lot easier on the tissues, especially if IHC is performed
on them later.
As far a an electronically created list of blocks going into a processor, I
haven't heard of any.
Hope this helps.
----- Original Message -----
From: "Lynn Wade" <lynnw <@t> nf.sympatico.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Sunday, March 23, 2008 6:31 PM
Subject: [Histonet] Reprocessing tissue
I am wondering if anyone has had an incident in Patholgy lab where on the
tissue processor the reagents got switched inadvertantly. For instance, the
80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus
water was reintroduced into the tissue just before xylene, clearing amd
Has anyone had this occur and how did you recover the tissue?
Also, can anyone tell me if there is such a processor that has a system that
can be used to log in the cassette numbers that are put onto the processor
so that in the event of some incident such as we had the retrieval of the
exact specimens can be done electronically?
And lastly can anyone tell me if they have a fully barcoded system whereby
path specimens arrive barcoded and every document, slide and block has a
barcode that allows for tracking of the tissue at all times?
We are looking at processes and trying to close some gaps.
Program Manager, Safety & Quality Management
Medical Services & Diagnostics
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