AW: [Histonet] Kidney Bx question

kemlo kemlo <@t>
Fri Mar 21 04:03:25 CDT 2008

If you are ever in Weston Super Mare then make for the boatyard in Uphill;
there's a small Hospital just before the boatyard less than a mile from the
beach. Come to reception ask for Kemlo, General Manager, they'll get me and
we can go for a pint. 

Look up Weston Super Mare in Google!!! Sea goes out to Wales (whales), beach
is composed of a bit of sand and Estuary mud, there are donkeys on the beach
and the Welsh try and walk home get stuck in the mud and drown; there's a
tractor in there somewhere!!! 

You can have a beer or a cider if you want; North Somerset specialises in
paint stripper called cider at about 50p a pint (fraid that's a dollar for a
little more than a US pint). Or I could come to Bend Oregon, but I bet the
beer's cold and fizzy and you don't have cider!!!

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Thomas
Sent: 20 March 2008 17:12
To: Marshall Terry Dr, Consultant Histopathologist
Cc: histonet <@t>
Subject: RE: AW: [Histonet] Kidney Bx question

Godfrey, Terry, Kemlo et al,

I believe it was Eleanor Roosevelt who said, "No one can make you feel bad
(unhappy, inferior, insert whatever negative word you like here) unless you
allow them to!"  I may not have the quote down exactly as stated but that is
the gist of it.  And unfortunately the world runs on perceptions.  People
are going to take things in whatever way they are perceived.  This of course
leads to misunderstandings, ruffled feathers etc.
So, I must say Godfrey, I've been watching and posting the histonet for some
time.  I have followed many a thread involving Terry and Kemlo. They are
both great resources, observant, knowledgable and yes...most of the time
doing their best to inject levity whenever possible.  And some folks don't
get on with that.  Not yet, having had the pleasure of personally meeting
either of them, I think I can safely say that in no way would either of them
have meant a comment to be an insult of any degree.
I believe many people use humor as a way of looking at problems and then
trying to determine solutions.  Sometimes it is necessary to point something
obvious (in this case TAT vs. quality) for the sake of bean counters, upper
administrators or any other party that influences our work lives and yet
doesn't fully understand where we're coming from.
I am truly sorry if you feel this whole thing was insulting.  I assure you I
would not have.  And please understand I'm not trying to apologize for
anyone here, I'm just interested in fostering better understanding.

Have a great day!

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Marshall
Terry Dr, Consultant Histopathologist
Sent: Thursday, March 20, 2008 7:32 AM
To: Godfrey Guerzon; histoinfo <@t>; Gudrun Lang
Cc: histonet <@t>
Subject: RE: AW: [Histonet] Kidney Bx question

Nobody has said anything snide, or put anybody down.
Your post is typical of those lacking a sense of humour, and who think being
professional is synonymous with being formal.
It is not.


-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Godfrey
Sent: 20 March 2008 14:17
To: histoinfo <@t>; Gudrun Lang
Cc: histonet <@t>
Subject: Re: AW: [Histonet] Kidney Bx question

We are supposed to be professionals.  What we post are real issues in our
situation and ask for help.  Professionals don't make snide remarks and put
down our colleagues.  If we think that it will raise our stature as
professionals when we make a joke and condescending comments on things that
we need help on, we are greatly mistaken. A wise man once said "if you have
nothing good to say about anything - shut up".  We need to understand that
each situation is different - who are we to judge?  We post a real issue in
our own situation and we don't need "smart" colleagues make a joke of our
situation. Do we want to be treated as professionals - then let us
communicate as professionals.

No snide remarks or condescending comments - PLEASE!!! - Help and good ideas
are welcome - if you have nothiing to contribute to the issue at hand -
please don't waste your (and ours) valuable time with useless comments.


>>> "Gudrun Lang" <gu.lang <@t>> 3/20/2008 9:53 AM >>>
Would you be so kind and explain, why you have to do a short run. Are these
indeed NTX-biopsies, that need a report as fast as possible?
Or is it just the fear to over-process the biopsies?

We have ntx from time to time, but even then, the clinicians wait for the
report until the next day, noon. So we can process the biopsies with our
regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG =
kind of trichrome), immunohistochemistry and immunofluorescence. 
We process the needlebiopsies in sponges and with the regular 13-hour
protocol. Regarding IHC a fixation-time under 6 hours could also drive to

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von
histoinfo <@t>
Gesendet: Donnerstag, 20. März 2008 14:01
An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist;
histonet <@t>
Betreff: RE: [Histonet] Kidney Bx question

Yes they are agitated. And the tearing problem that I originally described
is fairly infrequent. It's just that when it does happen we can't figure out
why. When it does happen nothing that we can see is any different than any
other day. Nothing in common as far as collection site either. I am
beginning to think more and more that it may be on days that we have 5 or 6
bxs that are all run together. But even then it is not every bx. I think we
will take better steps to prevent solution carryover and see what happens.
Of course it is so infrequent that we have this problem that it will be
months before we can say that we "fixed" the problem. I would still like to
compare times with others out there though. So please share your times for
hand processing.

-------------- Original message --------------
From: Rene J Buesa <rjbuesa <@t>> 

For hand processing at room temperature that schedule is too short, unless
the samples are agitated.
René J.

"Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t>> wrote:
Do you have a train to catch?


-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of
histoinfo <@t>
Sent: 19 March 2008 17:14
To: histonet <@t>
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules
looking torn look to the tissue. We hand process all our kidney bx's with
the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E &

We are trying to determine if this tearing is an artifact from processing,
collection or some other factor we haven't thought of. Has anyone else come
across this or a similar problem. All thoughts and suggestions welcome.

Jennifer Saunders HT (ASCP)
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