AW: [Histonet] Kidney Bx question

Robyn Vazquez vazquezr <@t>
Thu Mar 20 13:31:17 CDT 2008

Thomas well said!!! And excuse me, but I have a light rail to catch...:D+===
Portland, OR

>>> "Thomas Jasper" <tjasper <@t>> 3/20/2008 10:12 AM >>>

Godfrey, Terry, Kemlo et al,

I believe it was Eleanor Roosevelt who said, "No one can make you feel bad (unhappy, inferior, insert whatever negative word you like here) unless you allow them to!"  I may not have the quote down exactly as stated but that is the gist of it.  And unfortunately the world runs on perceptions.  People are going to take things in whatever way they are perceived.  This of course leads to misunderstandings, ruffled feathers etc.
So, I must say Godfrey, I've been watching and posting the histonet for some time.  I have followed many a thread involving Terry and Kemlo. They are both great resources, observant, knowledgable and yes...most of the time doing their best to inject levity whenever possible.  And some folks don't get on with that.  Not yet, having had the pleasure of personally meeting either of them, I think I can safely say that in no way would either of them have meant a comment to be an insult of any degree.
I believe many people use humor as a way of looking at problems and then trying to determine solutions.  Sometimes it is necessary to point something obvious (in this case TAT vs. quality) for the sake of bean counters, upper administrators or any other party that influences our work lives and yet doesn't fully understand where we're coming from.
I am truly sorry if you feel this whole thing was insulting.  I assure you I would not have.  And please understand I'm not trying to apologize for anyone here, I'm just interested in fostering better understanding.

Have a great day!

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701

-----Original Message-----
From: histonet-bounces <@t> [mailto:histonet-bounces <@t>] On Behalf Of Marshall Terry Dr, Consultant Histopathologist
Sent: Thursday, March 20, 2008 7:32 AM
To: Godfrey Guerzon; histoinfo <@t>; Gudrun Lang
Cc: histonet <@t>
Subject: RE: AW: [Histonet] Kidney Bx question

Nobody has said anything snide, or put anybody down.
Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal.
It is not.


-----Original Message-----
From: histonet-bounces <@t> [mailto:histonet-bounces <@t>] On Behalf Of Godfrey Guerzon
Sent: 20 March 2008 14:17
To: histoinfo <@t>; Gudrun Lang
Cc: histonet <@t>
Subject: Re: AW: [Histonet] Kidney Bx question

We are supposed to be professionals.  What we post are real issues in our situation and ask for help.  Professionals don't make snide remarks and put down our colleagues.  If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up".  We need to understand that each situation is different - who are we to judge?  We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals.

No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments.


>>> "Gudrun Lang" <gu.lang <@t>> 3/20/2008 9:53 AM >>>
Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible?
Or is it just the fear to over-process the biopsies?

We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. 
We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems.

Gudrun Lang

Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von histoinfo <@t>
Gesendet: Donnerstag, 20. März 2008 14:01
An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet <@t>
Betreff: RE: [Histonet] Kidney Bx question

Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens.
Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing.

-------------- Original message --------------
From: Rene J Buesa <rjbuesa <@t>> 

For hand processing at room temperature that schedule is too short, unless the samples are agitated.
René J.

"Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t>> wrote:
Do you have a train to catch?


-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of histoinfo <@t>
Sent: 19 March 2008 17:14
To: histonet <@t>
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. 

We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome.

Jennifer Saunders HT (ASCP)
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