AW: [Histonet] Kidney Bx question

Thu Mar 20 10:33:10 CDT 2008

Hello, My name is Marissa Perez and I am a recruiter here with HCCI.  We specialize in lab staffing. Does anyone know of any facilities who are desperately in need of HTs? 

Best regards,

Marissa Perez
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Thursday, March 20, 2008 10:25 AM
To: Godfrey Guerzon; histoinfo <@t> comcast.net; Gudrun Lang
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: AW: [Histonet] Kidney Bx question

Amen Godfrey I was thinking the same thing.....

Jennifer,
I would extend the processing times by about 5 minutes each and I would add one more formalin...

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon
Sent: Thursday, March 20, 2008 9:17 AM
To: histoinfo <@t> comcast.net; Gudrun Lang
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: AW: [Histonet] Kidney Bx question

We are supposed to be professionals.  What we post are real issues in our situation and ask for help.  Professionals don't make snide remarks and put down our colleagues.  If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up".  We need to understand that each situation is different - who are we to judge?  We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals.

No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments.

Godfrey

>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 3/20/2008 9:53 AM >>>
Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible?
Or is it just the fear to over-process the biopsies?

We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. 
We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems.

Gudrun Lang

Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von histoinfo <@t> comcast.net
Gesendet: Donnerstag, 20. März 2008 14:01
An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Kidney Bx question

Rene,
Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens.
Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing.
Thanks,
Jennifer

-------------- Original message --------------
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

For hand processing at room temperature that schedule is too short, unless the samples are agitated.
René J.

"Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk> wrote:
Do you have a train to catch?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histoinfo <@t> comcast.net
Sent: 19 March 2008 17:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. 

We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome.

Jennifer Saunders HT (ASCP)
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