[Histonet] paraformaldehyde autofluorescence

[Helen Beard]

We have been stirring pfa overnight @RT to dissolve, avoiding heat, as one of our researchers believes heating to 60-65C causes autofluorescence in tissue.  Has anyone had this problem with autofluorescence? I would prefer to heat and add NaOH. Also what are thoughts on using fresh, frozen 4%pfa?
Thanks in advance
 
Helen Beard
Adelaide South Australia
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