[Histonet] Removing DPX coverslips can you leave in xylene for several days without affecting tissue

[Pamela Marcum]

Yes!  You can remove by soaking until they come off with no problem in xylene.

Pam Marcum

At 01:43 PM 3/14/2008, Webber, Mark wrote:
>Have done DAB and then coverslipped with DPX I have been required to
>counterstain with haematoxylin. Can the coverslips be removed by soaking
>in Xylene.
>
>
>
>For reasons out of my control the sections may be in Xylene for several
>days
>
>
>
>Firstly will this affect the tissue?
>
>Secondly what would I need to do to counterstain (rehydrate/dehydrate
>etc??) as have never had to do this
>
>
>
>Thanks
>
>Mark
>
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Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu