[Histonet] Removing DPX coverslips can you leave in xylene for several days without affecting tissue

Webber, Mark mark.webber <@t> nuigalway.ie
Fri Mar 14 12:43:57 CDT 2008

Have done DAB and then coverslipped with DPX I have been required to
counterstain with haematoxylin. Can the coverslips be removed by soaking
in Xylene.


For reasons out of my control the sections may be in Xylene for several


Firstly will this affect the tissue? 

Secondly what would I need to do to counterstain (rehydrate/dehydrate
etc??) as have never had to do this




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