[Histonet] Lung processing

[louise renton]

Are you loading more cassettes into the new processor? are they
perhaps packed more tightly than before? - this may affect the effect
of vacuum on the "inner" cassettes in relation to those on the
outside.....
just my 2c worth

On 3/13/08, Weaver, Colin <c.weaver <@t> vla.defra.gsi.gov.uk> wrote:
> Hi - everyone we have just moved from routine processing in a VIP 1000
> to a Thermo-Shandon Excelsior. This has been working perfectly except
> when processing lung, especially spongy lung. When embedding after
> processing wax infiltration appears to be patchy, bubbles come out of
> the tissue when pressing the tissue into a mould, after trimming there
> are "holes" in the tissue, and sections often crumble because of lack of
> wax support. We have vacuumn on everything from first alcohol onwards.
> Anyone got any suggestions as to why this is happening? We used to use
> Thermo-Shandon Hypercenter XP's and had the same problem with lung, is
> it a Thermo thing?
>
> Colin
> Histology Dept
> VLA Thirsk
> Sunny Yorkshire
> Veterinary Laboratories Agency (VLA)
>
> This email and any attachments is intended for the named recipient
> only.
> If you have received it in error you have no authority to use, disclose,
> store or copy any of its contents and you should destroy it and inform
> the sender.
> Whilst this email and associated attachments will have been checked
> for known viruses whilst within VLA systems we can accept no
> responsibility once it has left our systems.
> Communications on VLA's computer systems may be monitored and/or
> recorded to secure the effective operation of the system and for other
> lawful purposes.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.