[Histonet] Slide Marker
Jerry Helisek
Jerry <@t> ralambusa.com
Thu Mar 13 11:41:52 CDT 2008
Helen, this may be overkill, but we manufacture a slide writer for ourselves (RA Lamb), ThermoFisher & TBS. See attached PDF.
Thanks,
------------------------------------
Raymond A. Lamb, Inc
Jerry Helisek
VP North America
jerry <@t> ralambusa.com
5409 Lumley Road, Unit 102
Durham, North Carolina 27703
tel: 919.957.1964
fax: 919.957.1972
mobile: 919.264.7964
Skype ID:jerryhelisek
------------------------------------
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, March 13, 2008 12:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 52, Issue 19
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Today's Topics:
1. prostate bx falling off slide (crochieresteve <@t> aol.com)
2. HT position available (crochieresteve <@t> aol.com)
3. Microwaves to keep or not to keep (Vacca Jessica)
4. S100P (Kimberly Tuttle)
5. Good Lab Practices (clarification) (Jenee.S.Odani <@t> hawaii.gov)
6. RE: Salary Survey for 2007-2008 (Ellenburg, Deborah)
7. Re: Good Lab Practices (clarification) (Rene J Buesa)
8. Re: prostate bx falling off slide (Rene J Buesa)
9. Doublecortin (Karen Lapanowski)
10. Textbook referrals (Celebre Julia)
11. Re: prostate bx falling off slide (Patricia Valente)
12. Digital Microscope Cameras for Classroom Use
(Ross Christian Langston)
13. MMA for Golgi? (Bob Nienhuis)
14. RE: Textbook referrals (Lee & Peggy Wenk)
15. Re: Doublecortin (Olek Michalski)
16. What type media? (Mary Lou Norman)
17. RE: Textbook referrals (Smith, Allen)
18. Re: Air bubbles (Emily Sours)
19. Gills III (Robyn Vazquez)
20. marker resistant to triton (Helen E Johnson)
21. Lung processing (Weaver, Colin)
22. Re: Air bubbles (Rene J Buesa)
23. Re: Gills III (Rene J Buesa)
24. Re: Lung processing (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Wed, 12 Mar 2008 13:08:25 -0400
From: crochieresteve <@t> aol.com
Subject: [Histonet] prostate bx falling off slide
To: histonet <@t> pathology.swmed.edu
Message-ID: <8CA527AD164F369-9A4-11D4 <@t> webmail-me06.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do??
Steve Crochiere
Histology Supervisor
Life Path Partners, Mercy Medical Center
------------------------------
Message: 2
Date: Wed, 12 Mar 2008 13:09:54 -0400
From: crochieresteve <@t> aol.com
Subject: [Histonet] HT position available
To: histonet <@t> pathology.swmed.edu
Message-ID: <8CA527B06CE9B3B-9A4-11E6 <@t> webmail-me06.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
We are looking for an experienced HT in the Springfield MA area to work in our hospital based laboratory and our satellite prostate lab. Send resume to Steve.crochiere <@t> SPHS.com
------------------------------
Message: 3
Date: Wed, 12 Mar 2008 13:52:27 -0400
From: "Vacca Jessica" <Jessica.Vacca <@t> HCAhealthcare.com>
Subject: [Histonet] Microwaves to keep or not to keep
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<41E16A15CE78374EA45B57E0F94339B8037DDCC8 <@t> ORLEV01.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
I would like to know how many of you are keeping your "household"
microwaves? I do a couple of special stains that we heat up the solutions. We don't do any processing with it. Those of you that are keeping them, what are you doing to avoid the ding? Or are you just taking your chances? Which do you recommend for a "lab" microwave?
Thanks
Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr.
Brandon Fl 33511
(813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca <@t> hcahealthcare.com
------------------------------
Message: 4
Date: Wed, 12 Mar 2008 13:57:58 -0400
From: "Kimberly Tuttle" <ktuttle <@t> umm.edu>
Subject: [Histonet] S100P
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <47D7E165.90CE.001A.3 <@t> umm.edu>
Content-Type: text/plain; charset=US-ASCII
Does anyone know where I can buy S100P, how much it costs, lead time.....Thanks
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail.
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Message: 5
Date: Wed, 12 Mar 2008 08:10:57 -1000
From: Jenee.S.Odani <@t> hawaii.gov
Subject: [Histonet] Good Lab Practices (clarification)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF76F643E8.E2C64CCD-ON0A25740A.006290A9-0A25740A.006535EB <@t> hawaii.gov>
Content-Type: text/plain; charset="US-ASCII"
I posted yesterday asking if anyone would share their protocols/manuals
regarding disposal of blocks/tissues.
For clarification, this is not because I need info to write a manual. Our
state does not have any clear guidelines, and a USDA official is trying to
get an idea of "what other labs do" to help certify a new lab in our
state.
Can you share with me how your lab disposes of blocks and tissues? What
logs or records are kept?
Jenee S. Odani, D.V.M., Dipl. ACVP
Veterinary Medical Officer
Hawaii State Veterinary Laboratory/DAI
99-941 Halawa Valley Street, Aiea, HI, 96701
Phone: (808) 483-7131/Fax: (808) 483-7110
------------------------------
Message: 6
Date: Wed, 12 Mar 2008 14:27:59 -0400
From: "Ellenburg, Deborah" <DEllenburg2 <@t> stfrancishealth.org>
Subject: [Histonet] RE: Salary Survey for 2007-2008
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EDEDD1F77BA8BC4BA1DA4B421762208201D9D037 <@t> EDC-MAIL-04.ads.bshsi.com>
Content-Type: text/plain; charset=iso-8859-1
Advance for Medical Laboratory Professionals is doing an online salary survey and are to have the results of the survey available in June.
You can logo to www.advanceweb.com/surveys to participate in the survey.
Hope this helps.
Debbie Ellenburg, HT (ASCP)
Histology Supervisor
Bon Secours St. Francis Health System
One St. Francis Drive
Greenville, SC 29601
864-255-1582
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, March 12, 2008 1:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 52, Issue 18
Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
1. Re: salery survey (Rene J Buesa)
2. Book for Fluorescent Staining (Bobrowitz, Carol)
3. Air bubbles (Karen Lapanowski)
4. CSH Symposium (Jennifer MacDonald)
5. RE: Air bubbles (Marshall Terry Dr, Consultant Histopathologist)
6. HMP300 tissue processor (Simon Smith)
7. RE: Book for Fluorescent Staining (Houston, Ronald)
----------------------------------------------------------------------
Message: 1
Date: Wed, 12 Mar 2008 07:11:03 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] salery survey
To: richard shook <rshooki_99 <@t> yahoo.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <326455.77544.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Under separate cover I am sending you my article (Annals of Diagnostic Pathology) on safety.
René J.
richard shook <rshooki_99 <@t> yahoo.com> wrote:
A quick question for all is there, or has there been a recent histology salery survey and if so where could i find it. i have seen one before but cant remember where it was
thank you
rich
rabkin derm path
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Message: 2
Date: Wed, 12 Mar 2008 10:39:57 -0500
From: "Bobrowitz, Carol" <cbobrowi <@t> mcw.edu>
Subject: [Histonet] Book for Fluorescent Staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8F78639AC56F4143B267FE5F5A1B92C8F0AEE0 <@t> guyton.phys.mcw.edu>
Content-Type: text/plain; charset="us-ascii"
Hi,
I am looking for a book or information booklet pertaining strictly to
fluorescent staining.
Something on the order of Dako' s immunohistochemical staining book or
C.M. vander Loos's multiple staining book.
Even a web page reference would be helpful.
I know Galye Callis is giving a workshop this summer in New Mexico,
unfortunately I can not attend.
Hopefully she will be giving this workshop in Pittsburg.
Any information that you can provide me will be greatly appreciated.
Thank you in advance for your help.
Carol Ann Bobrowitz
Department of Physiology
Medical College of Wisconsin
414-456-8179
cbobrowi <@t> mcw.edu
------------------------------
Message: 3
Date: Wed, 12 Mar 2008 11:16:58 -0400
From: "Karen Lapanowski" <KLAPANO1 <@t> hfhs.org>
Subject: [Histonet] Air bubbles
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20080312T111658Z_02DE000F0000 <@t> hfhs.org>
Content-Type: text/plain; charset=utf-8
Hello,
Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions?
Thanks,
Karen
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Message: 4
Date: Wed, 12 Mar 2008 09:26:30 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] CSH Symposium
To: histonet <@t> lists.utsouthwestern.edu
Cc: lfigueroa <@t> sakuraus.com
Message-ID:
<OF77854FE1.163851BB-ON8825740A.005A255E-8825740A.005A4653 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
The California Society for Histotechnology annual symposium will be held
at the Ventura Beach Marriott in Ventura, California. Please visit our
website for the complete program.
Thank you,
http://www.californiahistology.org/
Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu
------------------------------
Message: 5
Date: Wed, 12 Mar 2008 16:31:39 -0000
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Air bubbles
To: "Karen Lapanowski" <KLAPANO1 <@t> hfhs.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5C0BED61F529364E86309CADEA63FEF20163F387 <@t> TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
We are getting the same problem, except that it is (as yours probably
is) "dryback".
Terry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Karen
Lapanowski
Sent: 12 March 2008 15:17
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Air bubbles
Hello,
Air bubbles develop a week or so after coverslipping sections using
Vectashield hardset mounting media. (Initially, no air pockets were
visible.) Any suggestions?
Thanks,
Karen
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Message: 6
Date: Wed, 12 Mar 2008 09:32:07 -0700
From: "Simon Smith" <histion <@t> hotmail.com>
Subject: [Histonet] HMP300 tissue processor
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU127-DAV11ACF0C29A6759837A066DA1080 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Folks,
I have an old but good Microm/Hacker HMP300 tissue processor which is
refusing to behave at the moment.
When trying to run it fails at diagnostic #3, A more detailed check reveals
the problem to be a "Station valve driver fail off" error for station 14
(the xylene cleaning step). I have obtained and replaced the valve to no
effect, I swapped the electrical connections between station 14 and 15 (I
still get the same error for station 14) and am at the end of my patience
with the wee beastie.
I have the following questions:
1. What is causing the problem?
2. How do I fix it?
3. Is there anyone out there who remembers these machines and can fix
the problem? I am located just North of Seattle.
4. Failing that, does anyone have a service manual and/or parts I can
borrow or buy?
Thanks in advance for your help
Simon
------------------------------
Message: 7
Date: Wed, 12 Mar 2008 12:49:40 -0400
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: RE: [Histonet] Book for Fluorescent Staining
To: "Bobrowitz, Carol" <cbobrowi <@t> mcw.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<979FF5962E234F45B06CF0DB7C1AABB215E8CF1A <@t> chi2k3ms01.columbuschildrens.net>
Content-Type: text/plain; charset="us-ascii"
Protein Localization by Fluorescence Microscopy: a practical approach
Ed. VJ Allen
Pub OUP, 2000
ISBN: 0-19-963741-5 (hbk)
0-19-963740-7 (pbk)
Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Ronald.Houston <@t> NationwideChildrens.org
Columbus Children's Hospital is now Nationwide Children's Hospital
www.NationwideChildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bobrowitz, Carol
Sent: Wednesday, March 12, 2008 11:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Book for Fluorescent Staining
Hi,
I am looking for a book or information booklet pertaining strictly to
fluorescent staining.
Something on the order of Dako' s immunohistochemical staining book or
C.M. vander Loos's multiple staining book.
Even a web page reference would be helpful.
I know Galye Callis is giving a workshop this summer in New Mexico,
unfortunately I can not attend.
Hopefully she will be giving this workshop in Pittsburg.
Any information that you can provide me will be greatly appreciated.
Thank you in advance for your help.
Carol Ann Bobrowitz
Department of Physiology
Medical College of Wisconsin
414-456-8179
cbobrowi <@t> mcw.edu
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End of Histonet Digest, Vol 52, Issue 18
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Message: 7
Date: Wed, 12 Mar 2008 12:38:11 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Good Lab Practices (clarification)
To: Jenee.S.Odani <@t> hawaii.gov, histonet <@t> lists.utsouthwestern.edu
Message-ID: <200124.30165.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We used to incinerate them after keeping them for the prescribed time period.
René J.
Jenee.S.Odani <@t> hawaii.gov wrote:
I posted yesterday asking if anyone would share their protocols/manuals
regarding disposal of blocks/tissues.
For clarification, this is not because I need info to write a manual. Our
state does not have any clear guidelines, and a USDA official is trying to
get an idea of "what other labs do" to help certify a new lab in our
state.
Can you share with me how your lab disposes of blocks and tissues? What
logs or records are kept?
Jenee S. Odani, D.V.M., Dipl. ACVP
Veterinary Medical Officer
Hawaii State Veterinary Laboratory/DAI
99-941 Halawa Valley Street, Aiea, HI, 96701
Phone: (808) 483-7131/Fax: (808) 483-7110
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Message: 8
Date: Wed, 12 Mar 2008 12:42:54 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] prostate bx falling off slide
To: crochieresteve <@t> aol.com, histonet <@t> pathology.swmed.edu
Message-ID: <307439.49289.qm <@t> web65706.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Check your processing protocol. Maybe they are treated too long in dehydrant/clearant. How do they section, brittle? If that is the case it is an indicaiton of a too prolonged processing protocol.
René J.
crochieresteve <@t> aol.com wrote:
Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do??
Steve Crochiere
Histology Supervisor
Life Path Partners, Mercy Medical Center
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Message: 9
Date: Wed, 12 Mar 2008 16:24:40 -0400
From: "Karen Lapanowski" <KLAPANO1 <@t> hfhs.org>
Subject: [Histonet] Doublecortin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20080312T162440Z_02DE000F0000 <@t> hfhs.org>
Content-Type: text/plain; charset=utf-8
Hello,
What supplier do you use for doublecortin antibody? Will be staining paraffin sections using FITC as the secondary.
Thanks,
Karen Lapanowski
Karen Lapanowski
Radiation Oncology
3065 E & R
313-916-9386
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Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us.
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Message: 10
Date: Wed, 12 Mar 2008 16:41:13 -0400
From: "Celebre Julia" <celebrej <@t> HHSC.CA>
Subject: [Histonet] Textbook referrals
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4D3667D4B5487546A3139FB918181FEA078E34 <@t> ipemail01.hhsc.ca>
Content-Type: text/plain; charset="iso-8859-1"
Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available.
Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
905-527-0271 ext 46179
email: celebrej <@t> hhsc.ca
This information is directed in confidence solely to the person
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Message: 11
Date: Wed, 12 Mar 2008 13:47:41 -0700 (PDT)
From: Patricia Valente <pvalente <@t> sbcglobal.net>
Subject: Re: [Histonet] prostate bx falling off slide
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <447310.77002.qm <@t> web81703.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
After dewaxing the slides and taking down to water
try 10 minutes in 10%NBF, rinse well before retrieval.
Pat valente
uropathllc
--- crochieresteve <@t> aol.com wrote:
> Are there any suggestions to help keep prostate core
> biopsies on the plus slides when doing PIN-4 IHC. I
> use the Biocare de-cloaker and their BORG retrieval
> solution and Nemesis autostainer. I have been having
> a tough time with sections falling away after
> retrieval. I air dry them overnight and incubate in
> a 60 degree oven for 1 hour prior to de-wax and
> staining. What to do??
> Steve Crochiere
> Histology Supervisor
> Life Path Partners, Mercy Medical Center
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Pat
------------------------------
Message: 12
Date: Wed, 12 Mar 2008 10:55:53 -1000
From: Ross Christian Langston <langston <@t> hawaii.edu>
Subject: [Histonet] Digital Microscope Cameras for Classroom Use
To: Karen Lapanowski <KLAPANO1 <@t> hfhs.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <f5aafb9b5e43.47d7b6b9 <@t> hawaii.edu>
Content-Type: text/plain; charset=us-ascii
Hi Folks- could anyone with experience with either of the following two microscope cameras weigh-in on their merits or drawbacks? We are planning on projecting the feed onto a screen in our anatomy and physiology and lab techniques classes.
Thanks in advance for your input!
Ross
The cameras:
Q imaging Micropublisher 5.0 (uncooled)
Lumenera Infinity 1-1
Ross Langston, PhD
Department of Natural Sciences
Windward Community College
45-720 Keaahala Road
Kaneohe, Hawaii 96744
808-236-9119 (office)
808-247-5362 (fax)
----- Original Message -----
From: Karen Lapanowski <KLAPANO1 <@t> hfhs.org>
Date: Wednesday, March 12, 2008 10:26 am
Subject: [Histonet] Doublecortin
To: histonet <@t> lists.utsouthwestern.edu
> Hello,
> What supplier do you use for doublecortin antibody? Will be
> staining paraffin sections using FITC as the secondary.
> Thanks,
> Karen Lapanowski
>
> Karen Lapanowski
> Radiation Oncology
> 3065 E & R
> 313-916-9386
> ==============================================================================
> CONFIDENTIALITY NOTICE: This email contains information from the
> sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY
> or otherwise protected from disclosure. This email is intended for
> use only by the person or entity to whom it is addressed. If you
> are not the intended recipient, any use, disclosure, copying,
> distribution, printing, or any action taken in reliance on the
> contents of this email, is strictly prohibited. If you received
> this email in error, please contact the sending party by reply
> email, delete the email from your computer system and shred any
> paper copies.
>
> Note to Patients: There are a number of risks you should consider
> before using e-mail to communicate with us. See our Privacy Policy
> and Henry Ford My Health at www.henryford.com for more detailed
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> ==============================================================================
>
------------------------------
Message: 13
Date: Wed, 12 Mar 2008 14:47:47 -0800
From: "Bob Nienhuis" <bob.nienhuis <@t> gmail.com>
Subject: [Histonet] MMA for Golgi?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<45109da50803121547w1c69afb5k64fb11c61c61381b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Someone suggested the possibility of using MMA as an
embedding medium instead of celloidin for rapid Golgi.
How is it made soft enough for cutting with a microtome?
Anyone have experience with this, or have a protocol for
doing this?
Any thoughts or comments? I have looked at the Neil Hand
papers, but they don't seen to exactly apply to what I want
to do. I am looking to do thick sections so as to see full
dendritic arborization.
Bob Nienhuis
UCLA / VA Medical Center
Neurobiology Research
North Hills, CA 91343
------------------------------
Message: 14
Date: Thu, 13 Mar 2008 05:37:53 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] Textbook referrals
To: "'Celebre Julia'" <celebrej <@t> HHSC.CA>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000301c884ed$f1ab4c70$0202a8c0 <@t> HPPav2>
Content-Type: text/plain; charset="us-ascii"
NSH website has a list of books that are available and current, and were
suggested by Histonet members.
www.nsh.org
Highlight Career Center on left
Click on Tools and Resources
Click on Reference Materials
Books listed by topic:
- Histotechnology
- Management/Education
- Immunohistochemistry
- Molecular Pathology
- Histology Atlas
- Lab Math
- Lab Safety
- Misc. (skin, cytoprep)
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre
Julia
Sent: Wednesday, March 12, 2008 4:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Textbook referrals
Believe it or not, but there are funds available to us for purchasing new
Histology textbooks. I'm wondering if anyone in Histoland could kindly
suggest some good textbooks that are now available.
Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
905-527-0271 ext 46179
email: celebrej <@t> hhsc.ca
This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed. Therefore, this
information should be considered strictly confidential. If you have received
this email in error, please notify the sender immediately via a return email
for further direction. Thank you for your assistance.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 13 Mar 2008 13:34:10 +0100
From: "Olek Michalski" <olek.michalski <@t> nencki.gov.pl>
Subject: Re: [Histonet] Doublecortin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <op.t7yfu8pc445ael <@t> pc-313-1.ibd>
Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-2
I use Santa Cruz Biochemicals and the antibody (produced in goat) works
perfectly.
Best Regards
Olek Michalski
2008-03-12 21:24:40 Karen Lapanowski <KLAPANO1 <@t> hfhs.org> wrote:
> Hello,
> What supplier do you use for doublecortin antibody? Will be staining
> paraffin sections using FITC as the secondary.
> Thanks,
> Karen Lapanowski
>
> Karen Lapanowski
> Radiation Oncology
> 3065 E & R
> 313-916-9386
> ==============================================================================
> CONFIDENTIALITY NOTICE: This email contains information from the sender
> that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise
> protected from disclosure. This email is intended for use only by the
> person or entity to whom it is addressed. If you are not the intended
> recipient, any use, disclosure, copying, distribution, printing, or any
> action taken in reliance on the contents of this email, is strictly
> prohibited. If you received this email in error, please contact the
> sending party by reply email, delete the email from your computer system
> and shred any paper copies.
> Note to Patients: There are a number of risks you should consider before
> using e-mail to communicate with us. See our Privacy Policy and Henry
> Ford My Health at www.henryford.com for more detailed information. If
> you do not believe that our policy gives you the privacy and security
> protection you need, do not send e-mail or Internet communications to us.
>
> ==============================================================================
--
Laboratory of Neurobiology
of Development and Evolution
Nencki Institute of Experimental Biology
ul. Pasteura 3, 02-093 Warszawa, Poland
Tel. +48 22 5892268, Fax +48 22 8225342
------------------------------
Message: 16
Date: Thu, 13 Mar 2008 09:09:00 -0400
From: Mary Lou Norman <mlm11 <@t> cornell.edu>
Subject: [Histonet] What type media?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.1.2.2.20080313085627.03c0a720 <@t> postoffice9.mail.cornell.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Hello,
I don't do immuno so I am clueless. Please tell me the best media to grow
lung cells on so I can prepare them later for paraffin histopath.
H&E.
Thank you,
Mary Lou
------------------------------
Message: 17
Date: Thu, 13 Mar 2008 09:07:27 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: [Histonet] RE: Textbook referrals
To: 'Celebre Julia' <celebrej <@t> HHSC.CA>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D5304266F6C47274 <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
HISTOLOGY: A TEXT AND ATLAS, 5th ed. by Michael Ross and Wojciech Pawlina (long and detailed)
WHEATER'S FUNCTIONAL HISTOLOGY, 4th ed. by Barbara Young (very concise)
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre Julia
Sent: Wednesday, March 12, 2008 3:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Textbook referrals
Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available.
Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
905-527-0271 ext 46179
email: celebrej <@t> hhsc.ca
This information is directed in confidence solely to the person
named above and may not otherwise be distributed, copied or
disclosed. Therefore, this information should be considered
strictly confidential. If you have received this email in error, please
notify the sender immediately via a return email for further
direction. Thank you for your assistance.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Thu, 13 Mar 2008 09:51:39 -0400
From: "Emily Sours" <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Air bubbles
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b39794b0803130651y2b627ae7j3db6c4eccc0cc9c1 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
This may sound kind of stupid, but I always thought it might be
because more mounting media was needed when coverslipping. A lab down
the hall used the same protocol as ours and always had air bubbles
after a week. They only used two drops of media for coverslipping,
but we use four to five.
Emily
--
People aren't like chocolates. People are bastards. Bastards with
bastard coating and bastard filling.
------------------------------
Message: 19
Date: Thu, 13 Mar 2008 07:04:02 -0700
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: [Histonet] Gills III
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <s7d8d1f4.079 <@t> ohsu.edu>
Content-Type: text/plain; charset=us-ascii
Hello,
We have been using Gills III, as of recently approx 1 month, to do
frozens on a linear stainer, it has been getting stringy. Not a lot but
significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be
too old? I tried filtering it, but still stringy. I also, turned up
the water to help rinse it, but I don't want to take a chance on losing
tissue. Thanks in advanced.
Robyn
OHSU
------------------------------
Message: 20
Date: Thu, 13 Mar 2008 10:09:32 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] marker resistant to triton
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFAB81452B.9085BB31-ON8525740B.004D8C0C-8525740B.004DCA0E <@t> notes.health.state.ny.us>
Content-Type: text/plain; charset=US-ASCII
Hi Histonetters,
I am inquiring if there is a permanent slide marker that is resistant
to the chemical triton when doing IHC.
Helen Johnson
(hej01 <@t> health.state.ny.us)
IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation.
------------------------------
Message: 21
Date: Thu, 13 Mar 2008 15:00:49 -0000
From: "Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk>
Subject: [Histonet] Lung processing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7A885E8FE1C71C488D974EC601FAA69002E0865A <@t> vla-exchn1.cvlnt.vla.gov.uk>
Content-Type: text/plain; charset="us-ascii"
Hi - everyone we have just moved from routine processing in a VIP 1000
to a Thermo-Shandon Excelsior. This has been working perfectly except
when processing lung, especially spongy lung. When embedding after
processing wax infiltration appears to be patchy, bubbles come out of
the tissue when pressing the tissue into a mould, after trimming there
are "holes" in the tissue, and sections often crumble because of lack of
wax support. We have vacuumn on everything from first alcohol onwards.
Anyone got any suggestions as to why this is happening? We used to use
Thermo-Shandon Hypercenter XP's and had the same problem with lung, is
it a Thermo thing?
Colin
Histology Dept
VLA Thirsk
Sunny Yorkshire
Veterinary Laboratories Agency (VLA)
This email and any attachments is intended for the named recipient
only.
If you have received it in error you have no authority to use, disclose,
store or copy any of its contents and you should destroy it and inform
the sender.
Whilst this email and associated attachments will have been checked
for known viruses whilst within VLA systems we can accept no
responsibility once it has left our systems.
Communications on VLA's computer systems may be monitored and/or
recorded to secure the effective operation of the system and for other
lawful purposes.
------------------------------
Message: 22
Date: Thu, 13 Mar 2008 08:24:09 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Air bubbles
To: Emily Sours <talulahgosh <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <807810.16869.qm <@t> web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Emily:
That does not sound stupid, less mounting medium than required cause bubbles formation, as well as too tick mounting medium.
René J.
Emily Sours <talulahgosh <@t> gmail.com> wrote:
This may sound kind of stupid, but I always thought it might be
because more mounting media was needed when coverslipping. A lab down
the hall used the same protocol as ours and always had air bubbles
after a week. They only used two drops of media for coverslipping,
but we use four to five.
Emily
--
People aren't like chocolates. People are bastards. Bastards with
bastard coating and bastard filling.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Never miss a thing. Make Yahoo your homepage.
------------------------------
Message: 23
Date: Thu, 13 Mar 2008 08:26:24 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Gills III
To: Robyn Vazquez <vazquezr <@t> ohsu.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <605838.80002.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Opening a hematoxylin 3 months after receiving it cannot be the cause UNLESS it has an expiration date within that period, or is close to expiring (lets say before 2008).
René J.
Robyn Vazquez <vazquezr <@t> ohsu.edu> wrote:
Hello,
We have been using Gills III, as of recently approx 1 month, to do
frozens on a linear stainer, it has been getting stringy. Not a lot but
significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be
too old? I tried filtering it, but still stringy. I also, turned up
the water to help rinse it, but I don't want to take a chance on losing
tissue. Thanks in advanced.
Robyn
OHSU
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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------------------------------
Message: 24
Date: Thu, 13 Mar 2008 09:09:48 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Lung processing
To: "Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <380780.53437.qm <@t> web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Colin:
You hit "the nail in the head". It is "vacuum thing", I don't know if it is a "Thermo thing".
Check their operating manual and find out the vacuum level (mm of Hg) and compare with what the VIP 1000 used to have. If the vacuum is higher in the VIP 1000, that is your answer, if it is the same, then it has to be a "Thermo thing".
René J.
"Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk> wrote:
Hi - everyone we have just moved from routine processing in a VIP 1000
to a Thermo-Shandon Excelsior. This has been working perfectly except
when processing lung, especially spongy lung. When embedding after
processing wax infiltration appears to be patchy, bubbles come out of
the tissue when pressing the tissue into a mould, after trimming there
are "holes" in the tissue, and sections often crumble because of lack of
wax support. We have vacuumn on everything from first alcohol onwards.
Anyone got any suggestions as to why this is happening? We used to use
Thermo-Shandon Hypercenter XP's and had the same problem with lung, is
it a Thermo thing?
Colin
Histology Dept
VLA Thirsk
Sunny Yorkshire
Veterinary Laboratories Agency (VLA)
This email and any attachments is intended for the named recipient
only.
If you have received it in error you have no authority to use, disclose,
store or copy any of its contents and you should destroy it and inform
the sender.
Whilst this email and associated attachments will have been checked
for known viruses whilst within VLA systems we can accept no
responsibility once it has left our systems.
Communications on VLA's computer systems may be monitored and/or
recorded to secure the effective operation of the system and for other
lawful purposes.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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End of Histonet Digest, Vol 52, Issue 19
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