[Histonet] MMA for Golgi?

Bob Nienhuis bob.nienhuis <@t> gmail.com
Wed Mar 12 17:47:47 CDT 2008


Someone suggested the possibility of using MMA as an
embedding medium instead of celloidin for rapid Golgi.
How is it made soft enough for cutting with a microtome?

Anyone have experience with this, or have a protocol for
doing this?

Any thoughts or comments? I have looked at the Neil Hand
papers, but they don't seen to exactly apply to what I want
to do. I am looking to do thick sections so as to see full
dendritic arborization.

Bob Nienhuis
UCLA / VA Medical Center
Neurobiology Research
North Hills, CA 91343


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