Fw: Fw: [Histonet] The Leica Polaris

Anthony Reilly Tony_Reilly <@t> health.qld.gov.au
Tue Mar 11 22:27:27 CDT 2008

I have been using a Peloris for about 4 years now with excellent results.  The point to understand is that the instrument will always use the cleanest/purist of each reagent type last so when 2 runs are going at the same time they will be competing for the same last alcohol, xylene etc.  You will run into problems if for example you try to start a 1 hour run two hours after a 4 hour run as it may be delayed due to a clash in choice of reagent.  If possible always start a shorter run before a longer run.
I have not had a problem with water carryover but I have noticed that when exclusively using the reagent pump in/pump out facility that there is a small amount of reagent which can not be removed by the pump which will over time reduce the purity of reagents, so it is worthwhile to occasionally remove the containers and tip out any residue before refilling.
Tony Reilly

Chief Scientist
Anatomical Pathology
Pathology Queensland
Princess Alexandra Hospital
Ipswich Rd, 
Woolloongabba Q 4102
Ph: 07 32402412
Fax:07 32402930
tony_reilly <@t> health.qld.gov.au
>>> "Rebecca Johnson" <raj <@t> bluemarble.net> 12/03/2008 12:57 pm >>>
This is from a fellow histo tech having problems with her Polaris
Thanks for all of your advice
----- Original Message ----- 
From: "rocky&venita" <venrock12 <@t> peoplepc.com>
To: "Rebecca Johnson" <raj <@t> bluemarble.net>
Sent: Tuesday, March 11, 2008 3:01 PM
Subject: Re: Fw: [Histonet] The Leica Polaris

> rebecca ask if you load alot of autopsy blocks if that dilutes the 
> dilutions......----Original Message-----
>>From: Rebecca Johnson <raj <@t> bluemarble.net>
>>Sent: Mar 10, 2008 8:18 PM
>>To: Venita Sheets <venrock12 <@t> peoplepc.com>
>>Subject: Fw: [Histonet] The Leica Polaris
>>----- Original Message ----- 
>>From: "Beth Delescavage" <bdelescavage <@t> cellnetix.com>
>>To: "Rebecca Johnson" <raj <@t> bluemarble.net>
>>Sent: Monday, March 10, 2008 10:50 AM
>>Subject: RE: [Histonet] The Leica Polaris
>>There seems to be a little bit of a delay when you load two runs if you
>>want them off at the same time.  If you load them at slightly different
>>times you should be ok.  We did not have water contamination but we were
>>using the wrong wax and had infiltration issues.  Hope this helps.
>>CellNetix Labs, Seattle WA
>>-----Original Message-----
>>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
>>Sent: Saturday, March 08, 2008 5:32 PM
>>To: histonet
>>Subject: [Histonet] The Leica Polaris
>>Has anyone had problems with loading two runs at one time?
>>Also water contamination problems?
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu 
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