[Histonet] Melanin pigment

James L Burchette burch007 <@t> mc.duke.edu
Fri Mar 7 14:42:47 CST 2008

Perform a Giemsa counter stain after the slides come off the stainer. The 
Giemsa will color the melanin pigment green.

Jim Burchette, HT(ASCP) QIHC
"A simple histotech from a little country hospital in North Carolina"

"Cynthia Robinson" <Robinsoc <@t> mercyhealth.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/07/2008 01:34 PM

"histonet" <histonet <@t> lists.utsouthwestern.edu>

[Histonet] Melanin pigment

Has anyone successfully removed melanin pigment prior to IHC staining with 
MART-1?  Currently we use Ventana I-View DAB and I have suggested 
switching to AEC for these cases but our paths don't want to spend the 
money for a second detection system.  Any suggestions?
Cindi Robinson HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
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