AW: [Histonet] Antibody for CD7 clone LP15

[Andi Kappeler]

Hi Patty

we have compared clone LP15 with clone CD7-272 on various lymphoid tissues
and bone marrow trephines. LP15 performed indeed better than CD7-272 (which
I never really found that good). HIER is required for LP15, we had the best
results with pressure cooker - citrate (better than microwave-citrate, -TE
(pH 9.0), -Urea-Tris, pH 9.5)). 
Hope this helps.

Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Patti
Loykasek
Gesendet: Donnerstag, 6. März 2008 17:40
An: histonet
Betreff: [Histonet] Antibody for CD7 clone LP15

Hi all. Is anyone using the antibody for CD7 clone LP15? I just found out
that clone 272 has been discontinued, and the new item is clone LP15. A
search on PubMed has turned up nothing thus far. The vendor says that LP15
is superior for detection in paraffin ( I wasn't having any trouble with
272!). At any rate, just shopping for new clones. Of course we'll have to
get it in & re-validate it ( in our spare time!).
As always, thanks for the help.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA




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