[Histonet] Alk Phos activity

Prior, Andrew Andrew.Prior <@t> Smith-Nephew.com
Fri Mar 7 05:35:01 CST 2008

Dear Histonetters,

I've had a request to do some enzyme histochemical staining to
demonstrate Alkaline Phosphatase activity in bone (femoral condyles).
>From reading through the literature it seems that this can only be done
in unfixed frozen sections. However we need to do this in wax or MMA
sections (due to the other stains being performed and to allow
comparisons to previous studies). 
-Is it possible to demonstrate Alkaline Phosphatase activity in wax or
resin sections?
-From what I have read it seems FFPE sections are out - Is it the
fixation or the processing that inactivates the enzyme?
-Are there any fixatives that don't completely destroy the enzyme

I know frozen sections would work, but this is not an option. I look
forward to hearing your suggestions.

Thanks in advance


Andrew Prior
Smith &Nephew Research Centre
York Science Park
YO10 5DF
Andrew.Prior <@t> smith-nephew.com
01904 824022 
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