[Histonet] Question

Min-Han Tan minhan.tan <@t> gmail.com
Thu Mar 6 07:57:24 CST 2008

Dear Histonetters,

Thanks in advance for your advice.

I am interested in a possible red blood cell membrane protein, and am trying
to stain it on a charged slide.

The cells float off after paraformaldehyde 4% fixation, but they are fine
after methanol fixation.

Unfortunately, I do not see much staining of my protein of interest with the
latter fixing method.

Does anyone have any experience fixing RBC smears on slides so that the RBCs
do not drift off? Thanks!


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