[Histonet] H&E control slide

Cheryl tkngflght <@t> yahoo.com
Wed Mar 5 11:21:00 CST 2008

Hi Angela-

You want to cover the full range of cell types and fixatives you'll be
running through the stain--so this may vary by lab.  If you use alternate
fixatives on your sm bowel bx like Hollandes, you'll want to get some
leftover fresh bowel from a resection or autopsy and keep a stock of this
fixed in the alternate primary. Include a formalin piece and any thing else
with a wide range of cell types so you can catch any issues with your
control before staining a rack.  

Skin with a piece of bowel (NBF or your alternate fixative--or both would be
better) covers the full range of demonstrable cell types and gives enough
information to be sure the pH is where it should be (no 'robins egg blue'
staining in the mucin). 

To complete the loop, make sure to document the standard in your procedure
manual and include the QC verification on everyone's proficiency check sheet
if it's not already there.

Hope this helps!


Cheryl R. Kerry, HT(ASCP), BA

Full Staff Inc.

Staffing Allied Health Professionals - One GREAT tech at a time.

281.852.9457 office

281.883.7704 cell

800.756.3309 fax and alternate phone

admin <@t> fullstaff.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Sent: Wednesday, March 05, 2008 10:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E control slide

What is the consensus on what tissue to use for an H&E control. 

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 

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