SPAM-LOW: RE: [Histonet] Washing out formalin fixation

[Douglas D Deltour]

Happy Friday!! Ohh wait, it is only Tuesday. :(

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, March 04, 2008 8:35 AM
To: Rittman, Barry R; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Washing out formalin fixation

Barry:
  You should not disagree BEFORE knowing what I was referring to. You should
at least given me the benefit of the doubt and think that I was referring to
a tissue COMPLETELY fixed.
  Disagreeing for the sake of disagreeing is unhealthy to your state of
mind.
  René J.

"Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu> wrote:
  Rene
I disagree.
Depends on what you call fixation.
If tissue is fixed for a short period of time (say 1-2 hours) then the bonds
that are formed are not permanent and can be broken by washing in running
tap water.
If however you fix for several hours the this becomes much more difficult
due to extensive cross linking.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, March 03, 2008 4:16 PM
To: Johnson, Teri; 'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: [Histonet] Washing out formalin fixation

You cannot "unfix" a formalin fixed tissue with running water. It does not
work that way.
René J.

"Johnson, Teri" wrote:
Last week, a researcher here asked me what the chemical mechanism was of
washing out the effects of formalin fixation on the tissues with running
water. In other words, how does it work? Anybody here know?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110


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