SPAM-LOW: RE: [Histonet] Washing out formalin fixation

Douglas D Deltour doug <@t> ppspath.com
Tue Mar 4 07:52:43 CST 2008


Happy Friday!! Ohh wait, it is only Tuesday. :(

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, March 04, 2008 8:35 AM
To: Rittman, Barry R; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] Washing out formalin fixation

Barry:
  You should not disagree BEFORE knowing what I was referring to. You should
at least given me the benefit of the doubt and think that I was referring to
a tissue COMPLETELY fixed.
  Disagreeing for the sake of disagreeing is unhealthy to your state of
mind.
  René J.

"Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu> wrote:
  Rene
I disagree.
Depends on what you call fixation.
If tissue is fixed for a short period of time (say 1-2 hours) then the bonds
that are formed are not permanent and can be broken by washing in running
tap water.
If however you fix for several hours the this becomes much more difficult
due to extensive cross linking.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, March 03, 2008 4:16 PM
To: Johnson, Teri; 'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: [Histonet] Washing out formalin fixation

You cannot "unfix" a formalin fixed tissue with running water. It does not
work that way.
René J.

"Johnson, Teri" wrote:
Last week, a researcher here asked me what the chemical mechanism was of
washing out the effects of formalin fixation on the tissues with running
water. In other words, how does it work? Anybody here know?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



---------------------------------
Looking for last minute shopping deals? Find them fast with Yahoo! Search.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
Looking for last minute shopping deals?  Find them fast with Yahoo! Search.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






More information about the Histonet mailing list