[Histonet] Platelets cytospin preparation

[Reuel Cornelia]

I am doing an IF double staining  on platelet markers, CD42c, PF4, CD61 and PSTPIP1 on a cytospin prepared slides. I need  help on the cytospin preparation on a clotted blood buffy coat not on a anticoagulated blood. If you can share me a protocol that you have been using will be appreciated. Thank you very much.

Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768

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