[Histonet] Quality assurance
Featherstone, Annette
AFeatherstone <@t> KaleidaHealth.Org
Mon Jun 30 06:59:22 CDT 2008
We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides.
Annette Featherstone Kaleida Health
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Saturday, June 28, 2008 13:02
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 55, Issue 41
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Today's Topics:
1. Quality Assurance Measures (Sarah Kolekamp)
2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C)
3. FlexStation 3 needs new home (Erin Curry)
4. MOHS job openings at Loyola University Medical Center
(JimR0712 <@t> comcast.net)
5. RE: slide cabinets, RA Lamb (Jerry Helisek)
6. HT Positions available (Fidgen, Laura L.)
7. IHC/ISH (Ryan Salazar)
8. slide holders for Lerner Linear Stainer (Barbara Albert)
----------------------------------------------------------------------
Message: 1
Date: Fri, 27 Jun 2008 13:17:33 -0400
From: "Sarah Kolekamp" <kolekams <@t> trinity-health.org>
Subject: [Histonet] Quality Assurance Measures
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4864E86D0200006100015432 <@t> nodcgwcluster_gw4_server.no.trinity-health.org>
Content-Type: text/plain; charset=UTF-8
Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect??
Thanks,
Sarah Kolekamp
Sarah Kolekamp HTL (ASCP)
Lead Histotechnologist
Saint Mary's Health Care
Phone Number (616) 752-6155
Fax Number (616) 774-0628
>>> <histonet-request <@t> lists.utsouthwestern.edu> 06/27/08 1:02 PM >>>
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Today's Topics:
1. IHC marker for M-phase cells (Jean-Martin Lapointe)
2. HMB45 antibody (MaryAnn Dixon)
3. Formalin Recyling (John Spair)
4. Re: HMB45 antibody (Jan Shivers)
5. RE: whole mount cryosections (Atoska Gentry)
6. whole mount cryosections (Stephen Peters M.D.)
7. Prisma Stainer/coverslipper (Rebecca Johnson)
8. Vacuum pumps (Jennifer Johnson)
9. RE: IHC marker for M-phase cells (Connolly, Brett M)
10. lab tek metal slide cabinets (Patsy Ruegg)
----------------------------------------------------------------------
Message: 1
Date: Thu, 26 Jun 2008 13:23:17 -0400
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
Subject: [Histonet] IHC marker for M-phase cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BEFD613BD39142499989F836556DDC833CFEE1 <@t> ACE.accellab.lan>
Content-Type: text/plain; charset="iso-8859-1"
Hi Brett,
at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others.
Good luck
Jean-Martin
__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
Vice-President, Pathologie
AccelLAB Inc
1635 Lionel-Bertrand, Boisbriand
QuΘbec, Canada J7H 1N8
tel:á 450-435-9482 ext.247
fax: 450-435-4795
jm.lapointe <@t> accellab.com
á
á
________________________________________________________________
Message: 6
Date: Thu, 26 Jun 2008 09:27:58 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] IHC marker for M-phase cells
To: <histonet <@t> lists.utsouthwestern.edu>, <ihcrg <@t> googlegroups.com>
Message-ID:
<63EA0607835FBA4689CEA9EA8B48269201306BD2 <@t> usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"
I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody.
The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products.
Anyone had experience with this??
Or can you recommend another M-phase specific antibody?
Many thanks,
Brett
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com
********************
------------------------------
Message: 2
Date: Thu, 26 Jun 2008 13:56:05 -0400
From: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>
Subject: [Histonet] HMB45 antibody
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DBF15F4A549B4C40BF354D65B63E9EA34787BD <@t> EXGVMCNETWORK.vetmed.ufl.edu>
Content-Type: text/plain; charset="US-ASCII"
Hi histonetters,
Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance.
MaryAnn Dixon BS
Biological Scientist
Anatomic Pathology
UF Veterinary Medical Center
(352) 392-2235 Ext. 4517
------------------------------
Message: 3
Date: Thu, 26 Jun 2008 10:53:23 -0700
From: "John Spair" <John.Spair <@t> multicare.org>
Subject: [Histonet] Formalin Recyling
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<61A9977919846C479389493BAE2517CA023760EC <@t> MHSEXMBX1.multicare.org>
Content-Type: text/plain; charset=us-ascii
Is anyone using the formalin recycling/filtration system sold by
Creative Waste Solutions?? If you are , please email me at:
john.spair <@t> multicare.org if you don't mind, I have some questions.
Thank you.
"MMS <multicare.org>" made the following annotations.
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Message: 4
Date: Thu, 26 Jun 2008 15:10:29 -0500
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] HMB45 antibody
To: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B22416A3C70A441FA6903468F92C6260 <@t> auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive003 <@t> umn.edu
----- Original Message -----
From: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 26, 2008 12:56 PM
Subject: [Histonet] HMB45 antibody
Hi histonetters,
Anyone out there successfully using a monoclonal HMB45 antibody on
canine tissues? I think I've read a dozen papers all with different
protocols and my eyes are about to pop out of my head! Currently I'm at
a 1:50 dilution with a pretreatment in a citrate buffer (pressure
cooker). I have my choice of a polymer based detection as well as a
biotinylated detection. Any help would be appreciated. Thanks in
advance.
MaryAnn Dixon BS
Biological Scientist
Anatomic Pathology
UF Veterinary Medical Center
(352) 392-2235 Ext. 4517
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 26 Jun 2008 16:52:01 -0500
From: Atoska Gentry <gentras <@t> vetmed.auburn.edu>
Subject: [Histonet] RE: whole mount cryosections
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <48640F81.7080301 <@t> vetmed.auburn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
hello, will someone with expertise in fresh frozen whole mount and/or
coronal primate brain sections please contact me with pointers on
requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section
standard size sections but now one of our PI's is considering trying
larger samples. Thanks, Atoska
------------------------------
Message: 6
Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT)
From: "Stephen Peters M.D." <petepath <@t> yahoo.com>
Subject: [Histonet] whole mount cryosections
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <567183.62199.qm <@t> web45114.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Atoska,
á
Our 50 x 30 mm well bar setá with 55 x 35 mm chucks may give a bit more
room to work. See item # 115 on this page:
http://pathologyinnovations.com/Price%20List.htm
á
Stephen
á
------------------------------
Message: 7
Date: Sat, 26 Jan 2008 22:10:43 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Prisma Stainer/coverslipper
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <013d01c86092$341481b0$7b48f9d8 <@t> CHURCH>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Is anyone using the Prisma coverslipper in Cytology for the Imager? If
so
how are you doing it? Length of film and level.
Thanks
Becky
------------------------------
Message: 8
Date: Fri, 27 Jun 2008 09:32:59 -0400
From: Jennifer Johnson <jmjohnson34 <@t> hotmail.com>
Subject: [Histonet] Vacuum pumps
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU131-W2304949634EDDDDD2F5ACDBEA20 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
The James Watson Company sells new & used vacuum pumps. Their phone
number is
(770) 422-1154. I do not know if they have a web site. They are a
direct source instead of having to buy from a medical supply company
that will mark up your product.
Jennifer Johnson, HTL (ASCP)
P.S. I have an affiliation with this company. It is owned by my ex
brother-in-law twice removed.
_________________________________________________________________
The other season of giving begins 6/24/08. Check out the iÆm Talkathon.
http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving
------------------------------
Message: 9
Date: Fri, 27 Jun 2008 09:42:28 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] RE: IHC marker for M-phase cells
To: <histonet <@t> lists.utsouthwestern.edu>, <ihcrg <@t> googlegroups.com>
Message-ID:
<63EA0607835FBA4689CEA9EA8B48269201306E3A <@t> usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"
Thanks to all who replied to my question. Seems like everyone uses
anti-phosph-histone H3 (Ser10) as I have also used.
However, the Ser10 antibody has also been shown to label interphase G2
cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I
was really looking for an M-phase only marker. So if anyone has used the
phospho-Ser28 histone H3 antibody I would be interested to hear your
experiences.
Brett
> _____________________________________________
> From: Connolly, Brett M
> Sent: Thursday, June 26, 2008 9:28 AM
> To: histonet <@t> lists.utsouthwestern.edu; ihcrg <@t> googlegroups.com
> Subject: IHC marker for M-phase cells
>
> I am looking for an antibody for labeling FFPE sections of mitotic
> cells that are only in M-phase. Found one article in J Histo Cyto
> using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good,
> but the Authors produced their own antibody.
>
> The commercial suppliers I have been able to locate do not indicate
> IHC as a suitable application for their products.
>
> Anyone had experience with this??
>
> Or can you recommend another M-phase specific antibody?
>
> Many thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Research Fellow, Imaging Research
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> PH 215-652-2501 fax. 215-993-6803
> e-mail. brett_connolly <@t> merck.com
>
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Message: 10
Date: Fri, 27 Jun 2008 10:17:17 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] lab tek metal slide cabinets
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <005601c8d871$45f86570$6401a8c0 <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
I have some brand new lab tek metal slide cabinets that are missing the
drawer dividers, does anyone know where I can buy these? They turn the
drawer into two so you can line up slides or blocks side by side,
without
these nothing fits in the drawer.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
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End of Histonet Digest, Vol 55, Issue 40
****************************************
------------------------------
Message: 2
Date: Fri, 27 Jun 2008 12:10:48 -0700
From: "R C" <ruebenjcarter <@t> gmail.com>
Subject: [Histonet] IHC Marker for differentiation of Human to Mouse
hepatocytes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2a926e3f0806271210i5c91c94w131a39870892e9f8 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Is there a known Ab that exhibits + expression of hepatacytes in humans but
not mice?
Thank you.
Ruben Carter
------------------------------
Message: 3
Date: Fri, 27 Jun 2008 15:50:49 -0700
From: "Erin Curry" <erin <@t> aaisolutions.com>
Subject: [Histonet] FlexStation 3 needs new home
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com>
Content-Type: text/plain; charset="us-ascii"
Hello all,
We are looking for a new home for our Molecular Devices FlexStation 3
multimode microplate reader. It was new in 2006 and has liquid transfer
capabilities. If anyone is interested please contact me at the email
address below.
Erin Curry
1460 O'Brien Drive
Menlo Park, CA 94025
(650) 324-2569 erin <@t> aaisolutions.com
------------------------------
Message: 4
Date: Sat, 28 Jun 2008 00:16:26 +0000
From: JimR0712 <@t> comcast.net
Subject: [Histonet] MOHS job openings at Loyola University Medical
Center
To: histonet <@t> lists.utsouthwestern.edu (Histonet2),
histonet-request <@t> lists.utsouthwestern.edu (Histonet)
Message-ID:
<062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6 <@t> comcast.net>
Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409.
Jim Robinson, M.S., HTL/HT (ASCP)
Manager, Anatomic Pathology
------------------------------
Message: 5
Date: Fri, 27 Jun 2008 20:43:45 -0400
From: "Jerry Helisek" <Jerry <@t> ralambusa.com>
Subject: [Histonet] RE: slide cabinets, RA Lamb
To: <pruegg <@t> ihctech.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3855F92002259948A66A8CA2D16E3A4F0B5624 <@t> server.ralambusa.com>
Content-Type: text/plain; charset="us-ascii"
Patsy,
RA Lamb has drawer dividers that would work if you do not have any luck
finding those.
Thanks and have a great weekend.
------------------------------------
Raymond A. Lamb, Inc
Jerry Helisek
VP North America
jerry <@t> ralambusa.com
5409 Lumley Road, Unit 102
Durham, North Carolina 27703
tel: 919.957.1964
fax: 919.957.1972
mobile: 919.264.7964
Skype ID:jerryhelisek
------------------------------------
------------------------------
Message: 10
Date: Fri, 27 Jun 2008 10:17:17 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] lab tek metal slide cabinets
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <005601c8d871$45f86570$6401a8c0 <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
I have some brand new lab tek metal slide cabinets that are missing the
drawer dividers, does anyone know where I can buy these? They turn the
drawer into two so you can line up slides or blocks side by side,
without
these nothing fits in the drawer.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the
Person(s)
('the intended recipient') to whom it was addressed. Any views or
opinions
presented are solely those of the author. It may contain information
that is
privileged & confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message,
or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited.
If
you are NOT the intended recipient please contact the sender and dispose
of
this e-mail as soon as possible.
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End of Histonet Digest, Vol 55, Issue 40
****************************************
------------------------------
Message: 6
Date: Fri, 27 Jun 2008 17:46:09 -0700
From: "Fidgen, Laura L." <FidgenL <@t> shmc.org>
Subject: [Histonet] HT Positions available
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6BB8BC4519AAB844B174FC739A679BBC0148C025 <@t> IRMEXCH01.irm.inhs.org>
Content-Type: text/plain; charset="iso-8859-1"
Hello Histonetters!
Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191.
Laura Fidgen, HT(ASCP)cm
Histology Supervisor
Sacred Heart Medical Center
509-474-4106
------------------------------
Message: 7
Date: Sat, 28 Jun 2008 11:17:24 +0800
From: "Ryan Salazar" <Ryan.Salazar <@t> maccine.com>
Subject: [Histonet] IHC/ISH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EB9460BD9AB94D45A1B4C3FCFD2599E391CA58 <@t> MACSGSP2EX01.Maccine>
Content-Type: text/plain; charset="iso-8859-1"
Deareast Histopeeps!
We're currently upgrading our clinical research facility into the world of IHC and ISH.
Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures?
We're currently running the routine H&E and some special stains and we have the instruments needed for these.
We just want to be ready for our first IHC and ISH stuffs.
Thank you for your humble help and your big contribution!
Ryan S.
Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you.
------------------------------
Message: 8
Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT)
From: Barbara Albert <barbaraaalbert <@t> yahoo.com>
Subject: [Histonet] slide holders for Lerner Linear Stainer
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <994234.4916.qm <@t> web63707.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi all,
Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer. Well, we just moved and a box turned up. Let me know if you need them and these brand new slide holders are yours.
Barbara Albert
Lead Histotechnologist
UCSF Medical Center
San Francisco
415-353-1610
------------------------------
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End of Histonet Digest, Vol 55, Issue 41
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