[Histonet] Quality Assurance Measures
Sarah Kolekamp
kolekams <@t> trinity-health.org
Fri Jun 27 12:17:33 CDT 2008
Is anyone currently running a quality assurance program in their
histology lab, if so what do you measure/how? We are tring to develop
some standards and would like to have data to run analysis on...any
ideas on what data to collect??
Thanks,
Sarah Kolekamp
Sarah Kolekamp HTL (ASCP)
Lead Histotechnologist
Saint Mary's Health Care
Phone Number (616) 752-6155
Fax Number (616) 774-0628
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Today's Topics:
1. IHC marker for M-phase cells (Jean-Martin Lapointe)
2. HMB45 antibody (MaryAnn Dixon)
3. Formalin Recyling (John Spair)
4. Re: HMB45 antibody (Jan Shivers)
5. RE: whole mount cryosections (Atoska Gentry)
6. whole mount cryosections (Stephen Peters M.D.)
7. Prisma Stainer/coverslipper (Rebecca Johnson)
8. Vacuum pumps (Jennifer Johnson)
9. RE: IHC marker for M-phase cells (Connolly, Brett M)
10. lab tek metal slide cabinets (Patsy Ruegg)
----------------------------------------------------------------------
Message: 1
Date: Thu, 26 Jun 2008 13:23:17 -0400
From: "Jean-Martin Lapointe" <jm.lapointe <@t> accellab.com>
Subject: [Histonet] IHC marker for M-phase cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BEFD613BD39142499989F836556DDC833CFEE1 <@t> ACE.accellab.lan>
Content-Type: text/plain; charset="iso-8859-1"
Hi Brett,
at my previous job we did IHC staining for phospho-Histone H3 (ser10)
using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE
tissue, and it worked beautifully. It was on human origin tissue, don't
remember cross-reactivity in other species but Cell Signaling mentions
it reacts with many others.
Good luck
Jean-Martin
__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
Vice-President, Pathologie
AccelLAB Inc
1635 Lionel-Bertrand, Boisbriand
QuΘbec, Canada J7H 1N8
tel:á 450-435-9482 ext.247
fax: 450-435-4795
jm.lapointe <@t> accellab.com
á
á
________________________________________________________________
Message: 6
Date: Thu, 26 Jun 2008 09:27:58 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] IHC marker for M-phase cells
To: <histonet <@t> lists.utsouthwestern.edu>, <ihcrg <@t> googlegroups.com>
Message-ID:
<63EA0607835FBA4689CEA9EA8B48269201306BD2 <@t> usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"
I am looking for an antibody for labeling FFPE sections of mitotic cells
that are only in M-phase. Found one article in J Histo Cyto using clone
HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the
Authors produced their own antibody.
The commercial suppliers I have been able to locate do not indicate IHC
as a suitable application for their products.
Anyone had experience with this??
Or can you recommend another M-phase specific antibody?
Many thanks,
Brett
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com
********************
------------------------------
Message: 2
Date: Thu, 26 Jun 2008 13:56:05 -0400
From: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>
Subject: [Histonet] HMB45 antibody
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DBF15F4A549B4C40BF354D65B63E9EA34787BD <@t> EXGVMCNETWORK.vetmed.ufl.edu>
Content-Type: text/plain; charset="US-ASCII"
Hi histonetters,
Anyone out there successfully using a monoclonal HMB45 antibody on
canine tissues? I think I've read a dozen papers all with different
protocols and my eyes are about to pop out of my head! Currently I'm at
a 1:50 dilution with a pretreatment in a citrate buffer (pressure
cooker). I have my choice of a polymer based detection as well as a
biotinylated detection. Any help would be appreciated. Thanks in
advance.
MaryAnn Dixon BS
Biological Scientist
Anatomic Pathology
UF Veterinary Medical Center
(352) 392-2235 Ext. 4517
------------------------------
Message: 3
Date: Thu, 26 Jun 2008 10:53:23 -0700
From: "John Spair" <John.Spair <@t> multicare.org>
Subject: [Histonet] Formalin Recyling
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<61A9977919846C479389493BAE2517CA023760EC <@t> MHSEXMBX1.multicare.org>
Content-Type: text/plain; charset=us-ascii
Is anyone using the formalin recycling/filtration system sold by
Creative Waste Solutions?? If you are , please email me at:
john.spair <@t> multicare.org if you don't mind, I have some questions.
Thank you.
"MMS <multicare.org>" made the following annotations.
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------------------------------
Message: 4
Date: Thu, 26 Jun 2008 15:10:29 -0500
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] HMB45 antibody
To: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B22416A3C70A441FA6903468F92C6260 <@t> auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I had little or no success with HMB45 on canine tissue. I use Melan-A
exclusively; that works fine.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive003 <@t> umn.edu
----- Original Message -----
From: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 26, 2008 12:56 PM
Subject: [Histonet] HMB45 antibody
Hi histonetters,
Anyone out there successfully using a monoclonal HMB45 antibody on
canine tissues? I think I've read a dozen papers all with different
protocols and my eyes are about to pop out of my head! Currently I'm at
a 1:50 dilution with a pretreatment in a citrate buffer (pressure
cooker). I have my choice of a polymer based detection as well as a
biotinylated detection. Any help would be appreciated. Thanks in
advance.
MaryAnn Dixon BS
Biological Scientist
Anatomic Pathology
UF Veterinary Medical Center
(352) 392-2235 Ext. 4517
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 26 Jun 2008 16:52:01 -0500
From: Atoska Gentry <gentras <@t> vetmed.auburn.edu>
Subject: [Histonet] RE: whole mount cryosections
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <48640F81.7080301 <@t> vetmed.auburn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
hello, will someone with expertise in fresh frozen whole mount and/or
coronal primate brain sections please contact me with pointers on
requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section
standard size sections but now one of our PI's is considering trying
larger samples. Thanks, Atoska
------------------------------
Message: 6
Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT)
From: "Stephen Peters M.D." <petepath <@t> yahoo.com>
Subject: [Histonet] whole mount cryosections
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <567183.62199.qm <@t> web45114.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Atoska,
á
Our 50 x 30 mm well bar setá with 55 x 35 mm chucks may give a bit more
room to work. See item # 115 on this page:
http://pathologyinnovations.com/Price%20List.htm
á
Stephen
á
------------------------------
Message: 7
Date: Sat, 26 Jan 2008 22:10:43 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Prisma Stainer/coverslipper
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <013d01c86092$341481b0$7b48f9d8 <@t> CHURCH>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Is anyone using the Prisma coverslipper in Cytology for the Imager? If
so
how are you doing it? Length of film and level.
Thanks
Becky
------------------------------
Message: 8
Date: Fri, 27 Jun 2008 09:32:59 -0400
From: Jennifer Johnson <jmjohnson34 <@t> hotmail.com>
Subject: [Histonet] Vacuum pumps
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU131-W2304949634EDDDDD2F5ACDBEA20 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
The James Watson Company sells new & used vacuum pumps. Their phone
number is
(770) 422-1154. I do not know if they have a web site. They are a
direct source instead of having to buy from a medical supply company
that will mark up your product.
Jennifer Johnson, HTL (ASCP)
P.S. I have an affiliation with this company. It is owned by my ex
brother-in-law twice removed.
_________________________________________________________________
The other season of giving begins 6/24/08. Check out the iÆm Talkathon.
http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving
------------------------------
Message: 9
Date: Fri, 27 Jun 2008 09:42:28 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] RE: IHC marker for M-phase cells
To: <histonet <@t> lists.utsouthwestern.edu>, <ihcrg <@t> googlegroups.com>
Message-ID:
<63EA0607835FBA4689CEA9EA8B48269201306E3A <@t> usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"
Thanks to all who replied to my question. Seems like everyone uses
anti-phosph-histone H3 (Ser10) as I have also used.
However, the Ser10 antibody has also been shown to label interphase G2
cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I
was really looking for an M-phase only marker. So if anyone has used the
phospho-Ser28 histone H3 antibody I would be interested to hear your
experiences.
Brett
> _____________________________________________
> From: Connolly, Brett M
> Sent: Thursday, June 26, 2008 9:28 AM
> To: histonet <@t> lists.utsouthwestern.edu; ihcrg <@t> googlegroups.com
> Subject: IHC marker for M-phase cells
>
> I am looking for an antibody for labeling FFPE sections of mitotic
> cells that are only in M-phase. Found one article in J Histo Cyto
> using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good,
> but the Authors produced their own antibody.
>
> The commercial suppliers I have been able to locate do not indicate
> IHC as a suitable application for their products.
>
> Anyone had experience with this??
>
> Or can you recommend another M-phase specific antibody?
>
> Many thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Research Fellow, Imaging Research
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> PH 215-652-2501 fax. 215-993-6803
> e-mail. brett_connolly <@t> merck.com
>
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Message: 10
Date: Fri, 27 Jun 2008 10:17:17 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] lab tek metal slide cabinets
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <005601c8d871$45f86570$6401a8c0 <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
I have some brand new lab tek metal slide cabinets that are missing the
drawer dividers, does anyone know where I can buy these? They turn the
drawer into two so you can line up slides or blocks side by side,
without
these nothing fits in the drawer.
Thank you,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
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