[Histonet] IHC marker for M-phase cells

anh2006 <@t> med.cornell.edu anh2006 <@t> med.cornell.edu
Thu Jun 26 09:36:46 CDT 2008

Phospho histone H3 from upstate biotechnology is fabulous on FFPE tissue.

-----Original Message-----
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>

Date: Thu, 26 Jun 2008 09:27:58 
To:histonet <@t> lists.utsouthwestern.edu, ihcrg <@t> googlegroups.com
Subject: [Histonet] IHC marker for M-phase cells

I am looking for an antibody for labeling FFPE sections of mitotic cells
that are only in M-phase. Found one article in J Histo Cyto using clone
HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the
Authors produced their own antibody.

The commercial suppliers I have been able to locate do not indicate IHC
as a suitable application for their products.

Anyone had experience with this??  

Or can you recommend another M-phase specific antibody?

Many thanks,

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Research
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
PH 215-652-2501 fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com

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