[Histonet] NH4Cl

[Hazel Stevens]

It is used to quench autofluorescence of the tissue. 
Hazel 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, June 17, 2008 7:53 AM
To: William Connor; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] NH4Cl

Never heard of such a pretreatment with routine IHC protocols.
  René J.

William Connor <will <@t> histologytechservices.com> wrote:
  Greetings,



Could anyone tell me why a pretreatment of 50 mM NH4Cl would be used in
immunohistochemistry?



Thanks.





William P. Connor, HT(ASCP)

Senior Histology Technician

Histology Tech Services, Inc.



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