[Histonet] uneven HE staining

Atoska Gentry gentras <@t> vetmed.auburn.edu
Mon Jun 16 14:06:31 CDT 2008

Hello, when this type staining problem occurred in manual staining it 
was discovered to be caused by  incomplete deparaffinization.  
Corrective measure taken was to  replace clearing agents with fresh 
solution(s). However, I'm not familiar with the Tissue Tek Stainer or 
any of the other automated stainers. Best wishes. Atoska

Paula Wilder wrote:
> Hi everyone!
> A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer.  This has been an ongoing problem.  She tells me it is only on biopsies, very intermittent.  On one slide she might have four sections; three stain evenly and one does not.  The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last.  Several technicians are cutting so technique has been eliminated.  They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next?  We don't think it is the processing; dehydration?  I do not have their HE programmed times.
> Thanks for your help,
> Paula Wilder
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