[Histonet] RE: mouse T cell markers

[Milne, Katy]

Hi Christian, 

Depends on what you're looking for.  LabVision's CD3 rabbit monoclonal
is great as a pan T cell marker (Clone SP7).  E-bioscience has a good
FoxP3 RtMAb for Tregs (clone FJK-16).  Other than that it gets a little
sketchy finding good antibodies for mouse FFPE tissue.  I haven't had
any luck with other CD markers that I have been interested in (CD4, CD8
etc) looking through the archives, you'll probably find it's a fruitless
search!  If you would like granzyme B for activated cytolytic cells (and
NKs), we use one from Abcam (I think it's cat# ab4059 but I don't have
the vial in front of me).

Good luck!
Katy Milne
Deeley Research Centre
BC Cancer Agency


Message: 9
Date: Thu, 12 Jun 2008 13:33:45 -0700
From: Christian Franci <cfranci <@t> rigel.com>
Subject: [Histonet] mouse T cell markers
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <9c3f51cf44cf5eebea379806fbc97639 <@t> rigel.com>
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Hello, I was wondering if y'all had any thoughts on good anti- mouse T 
cell antibodies for paraffin IHC.
Thanx a bunch,
C