[Histonet] Mouse Mammary Gland

Land, Kimberly J KjLand <@t> usi.edu
Fri Jun 13 08:44:11 CDT 2008

Dear Histonetters,

  In the very near future, I will be preparing whole mounts of mouse
mammary glands.  The plan is to use Carnoy's fixative and to stain with
Carmine alum overnight.  That part of the procedure I think I can
handle.  My main concern is that afterwards, I am to embed in paraffin
for H&E staining.  Has anyone out there done this before??  Does the
Carmine alum interfere with the H&E?  How thick do the sections need to
be? I am used to histology on mouse thyroids, so this is a totally new
thing for me.  Any help would be appreciated.


Thank you,


Kim Lohe

University of Southern Indiana

8600 University Blvd.

Evansville, IN 47712


More information about the Histonet mailing list