[Histonet] RE: Histonet Digest, Vol 55, Issue 21

[Barbara Hale]

> From: histonet-request <@t> lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 55, Issue 21> To: histonet <@t> lists.utsouthwestern.edu> Date: Thu, 12 Jun 2008 10:00:52 -0700> > Send Histonet mailing list submissions to> histonet <@t> lists.utsouthwestern.edu> > To subscribe or unsubscribe via the World Wide Web, visit> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to> histonet-request <@t> lists.utsouthwestern.edu> > You can reach the person managing the list at> histonet-owner <@t> lists.utsouthwestern.edu> > When replying, please edit your Subject line so it is more specific> than "Re: Contents of Histonet digest..."> > > Today's Topics:> > 1. RE: Storage of Whole Mount Slides> (Marshall Terry Dr, Consultant Histopathologist)> 2. RE: Fite stain (Martin, Erin)> 3. Hematoxylin shortage (Martin, Erin)> 4. RE: Hematoxylin shortage (Mahoney,Janice A)> 5. Re: Storage of Whole Mount Slides (Jack Cates)> 6. Re: Slide Printer (Matt Bancroft)> 7. RE: Logging in same type specimens consecutively> (Della Speranza, Vinnie)> > > ----------------------------------------------------------------------> > Message: 1> Date: Thu, 12 Jun 2008 12:15:16 +0100> From: "Marshall Terry Dr, Consultant Histopathologist"> <Terry.Marshall <@t> rothgen.nhs.uk>> Subject: RE: [Histonet] Storage of Whole Mount Slides> To: <Pat.Bell <@t> UCHSC.edu>, <kenneth.metzger <@t> aruplab.com>,> <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <5C0BED61F529364E86309CADEA63FEF20163F425 <@t> TRFT-EX01.xRothGen.nhs.uk>> Content-Type: text/plain; charset="us-ascii"> > What use are whole mount slides now. Scan them and put them on a disc.> > Terry > > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of> Pat.Bell <@t> UCHSC.edu> Sent: 11 June 2008 21:03> To: kenneth.metzger <@t> aruplab.com; histonet <@t> lists.utsouthwestern.edu> Subject: RE: [Histonet] Storage of Whole Mount Slides> > Try VWR. I used to get slides boxes that size from them.> > Pat Bell HT(ASCP)> Sr. PRA> Division of Medical Oncology> UCHSC, Aurora, CO> 303-724-3845 (lab)> 303-724-3889 (fax)> > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Metzger,> Kenneth> Sent: Wednesday, June 11, 2008 11:55 AM> To: histonet <@t> lists.utsouthwestern.edu> Subject: [Histonet] Storage of Whole Mount Slides> > Does anyone know where to get storage drawers or boxes for 3 inch x 2> inch whole mount slides? Thanks,> > Ken> > Ken Metzger HTL(ASCP)> Histology Supervisor> ARUP Laboratories> 500 Chipeta way> Salt Lake City, UT 84108> 801.583.2787 ext 3101> > > - ------------------------------------------------------------------> The information transmitted by this e-mail and any included attachments> are from ARUP Laboratories and are intended only for the recipient. The> information contained in this message is confidential and may constitute> inside or non-public information under international, federal, or state> securities laws, or protected health information and is intended only> for the use of the recipient.> Unauthorized forwarding, printing, copying, distributing, or use of such> information is strictly prohibited and may be unlawful. If you are not> the intended recipient, please promptly delete this e-mail and notify> the sender of the delivery error or you may call ARUP Laboratories> Compliance Hot Line in Salt Lake City, Utah USA at (+1> (800) 522-2787 ext. 2100> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ------------------------------> > Message: 2> Date: Thu, 12 Jun 2008 06:08:36 -0700> From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>> Subject: RE: [Histonet] Fite stain> To: "Rena Fail" <renafail <@t> bellsouth.net>, "Tony Henwood"> <AnthonyH <@t> chw.edu.au>, "histonet" <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <DA617A88F598DE4888173E76E7E0783D025E458F <@t> EXVS07.net.ucsf.edu>> Content-Type: text/plain; charset=iso-8859-1> > I am using the American MasterTech kit and following their procedure... Do you think that changing to a sulfuric acid differentiation would remove the false postives in granulomatous infiltrate that the acid alcohol is apparently not removing?> > Thanks> Erin> > ________________________________> > From: Rena Fail [mailto:renafail <@t> bellsouth.net]> Sent: Sat 7/12/2008 4:39 PM> To: 'Tony Henwood'; Martin, Erin; 'histonet'> Subject: RE: [Histonet] Fite stain> > > > Acid alcohol will remove your dye from those bugs requiring the peanut> oil (usually Lepra Bacilli). It will also remove it from other acid fast> organisms, but at a slower rate. 1% sulfuric acid differentiation gives> more consistent results.> Rena Fail> > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony> Henwood> Sent: Wednesday, June 11, 2008 7:20 PM> To: Martin, Erin; histonet> Subject: RE: [Histonet] Fite stain> > Your differentiation step is used in the classic ZN not a Fite.> This might just be a typo but shouldn't it be 1-3% sulphuric acid (or> other acid). The bugs you are trying to demonstrate with the Fite stain> are acid fast but acid-alcohol labile.> > This won't solve the problem with you odd staining though> > Regards> > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)> Laboratory Manager & Senior Scientist> The Children's Hospital at Westmead,> Locked Bag 4001, Westmead, 2145, AUSTRALIA.> Tel: 612 9845 3306> Fax: 612 9845 3318> > > > > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,> Erin> Sent: Wednesday, 11 June 2008 10:57 PM> To: histonet> Subject: [Histonet] Fite stain> > > Good morning everyone, > > I am having a problem with my Fite stains. Most stain beautifully but I> have had trouble with a few cases. Here are the doc's comments:> > "case xxxx has some lipomembranous change- and this may not really be an> entirely false positive result. But there is clearly no explanation for> the stuff in xxxx that stains, including some fibrin. I've never seen> this before. It's not the usual round spots from inadequately rinsing> off the peanut oil...."> > "A couple of days ago, I saw a granulomatous reaction that had tiny Fite> positive "blobbos" that looked like microbes in tiny cystic spaces> within the granulomas. I initially thought they might be real organisms> but then I did a TB stain and proved that they were nothing. Just> moments ago, XXXX had a case that was essentially identical. The Fite> stain is working perfectly for ID of microbes, but it seems to have a> false positive kind of reactivity that can be seen in small spaces> within a granulomatous infiltrate. On an occasional basis only."> > Does anyone have any idea? Our procedure is 2 changes of xylene/peanut> oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in> 1% acid alcohol, rinse, counterstain in methylene blue, air dry.> > Thanks in advance,> > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > *********************************************************************> This email and any files transmitted with it are confidential and> intended solely for the use of the individual or entity to whom they are> addressed. If you are not the intended recipient, please delete it and> notify the sender.> > Views expressed in this message and any attachments are those of the> individual sender, and are not necessarily the views of The Children's> Hospital at Westmead> > This note also confirms that this email message has been> virus scanned and although no computer viruses were detected, The> Childrens Hospital at Westmead accepts no liability for any> consequential damage resulting from email containing computer viruses.> **********************************************************************> > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > ------------------------------> > Message: 3> Date: Thu, 12 Jun 2008 06:13:18 -0700> From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>> Subject: [Histonet] Hematoxylin shortage> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>,> september.amspacher <@t> bassett.org> Message-ID:> <DA617A88F598DE4888173E76E7E0783D025E4590 <@t> EXVS07.net.ucsf.edu>> Content-Type: text/plain; charset=iso-8859-1> > We also have been told that the shortage will last at least until the early autumn. Our pathologists are interested in the Phoenix Blue synthetic hematoxylin and we are supposed to be seeing some example slides next week. I can let you know what they think...> > > > > ------------------------------> > Message: 4> Date: Thu, 12 Jun 2008 08:43:14 -0500> From: "Mahoney,Janice A" <JMahoney <@t> alegent.org>> Subject: [Histonet] RE: Hematoxylin shortage> To: "'Martin, Erin'" <Erin.Martin <@t> ucsf.edu>, histonet> <histonet <@t> lists.utsouthwestern.edu>, "september.amspacher <@t> bassett.org"> <september.amspacher <@t> bassett.org>> Message-ID:> <346E5878979BA54FB4B0BFD6AD93B9B9B01F62339F <@t> EXCHMBC1.ad.ah.local>> Content-Type: text/plain; charset="us-ascii"> > We are in the process of working it up in my lab. So far so good. We do not have our staining times perfected yet but we think Phoenix blue has potential to be a great solution to the shortage.> I'll keep you informed of the progress.> Jan Mahoney> Omaha> > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin> Sent: Thursday, June 12, 2008 8:13 AM> To: histonet; september.amspacher <@t> bassett.org> Subject: [Histonet] Hematoxylin shortage> > We also have been told that the shortage will last at least until the early autumn. Our pathologists are interested in the Phoenix Blue synthetic hematoxylin and we are supposed to be seeing some example slides next week. I can let you know what they think...> > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person.> > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation.> > > > > ------------------------------> > Message: 5> Date: Thu, 12 Jun 2008 07:30:43 -0700 (PDT)> From: Jack Cates <pinetree110567 <@t> yahoo.com>> Subject: [Histonet] Re: Storage of Whole Mount Slides> To: histonet <@t> lists.utsouthwestern.edu> Message-ID: <544191.46545.qm <@t> web44912.mail.sp1.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1> > Ken,> We use a system for all of our storage from RA Lamb (although we purchase via PSL).  System is very nice and works great for the SuperMega cassettes as well.> Good luck on your search!!!> Jack> ------------------------------> > Message: 7> Date: Wed, 11 Jun 2008 11:54:34 -0600> From: "Metzger, Kenneth" <kenneth.metzger <@t> aruplab.com>> Subject: [Histonet] Storage of Whole Mount Slides> To: <histonet <@t> lists.utsouthwestern.edu>> Message-ID:>     <BB644A4EB18113488C431FC9D5E4584A01D0667A <@t> postoffice01.aruplab.net>> Content-Type: text/plain;    charset="iso-8859-1"> > Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks,> > Ken> > Ken Metzger HTL(ASCP)> Histology Supervisor> ARUP Laboratories> 500 Chipeta way> Salt Lake City, UT 84108> 801.583.2787 ext 3101> > > > > > ------------------------------> > Message: 6> Date: Thu, 12 Jun 2008 07:47:30 -0700 (PDT)> From: Matt Bancroft <mohs76009 <@t> yahoo.com>> Subject: Re: [Histonet] Slide Printer> To: rfields <@t> gidocs.net, Stephen.Clark1 <@t> hcahealthcare.com,> "histonet <@t> lists.utsouthwestern.edu"> <histonet <@t> lists.utsouthwestern.edu>, Amos Brooks> <amosbrooks <@t> gmail.com>> Message-ID: <158464.75392.qm <@t> web63404.mail.re1.yahoo.com>> Content-Type: text/plain; charset=us-ascii> > I use thermo slides and I do not have any tissue folowing off.&nbsp; I use them in mohs and in routine.> > --- On Wed, 6/11/08, Amos Brooks &lt;amosbrooks <@t> gmail.com&gt; wrote:> > From: Amos Brooks &lt;amosbrooks <@t> gmail.com&gt;> Subject: [Histonet] Slide Printer> To: rfields <@t> gidocs.net, Stephen.Clark1 <@t> hcahealthcare.com, "histonet <@t> lists.utsouthwestern.edu" &lt;histonet <@t> lists.utsouthwestern.edu&gt;> Date: Wednesday, June 11, 2008, 5:18 PM> > Rosa,> Good labeler concept, but it is a victim of a really bad proprietary> issue. It is unfortunate that the only company that I have found that makes> Colormark slides (or anything like them) is Erie Scientific. (Suggestions> anyone?) They have terrible quality control. Tissue is constantly falling> off our slides (yes, the expensive charged ones) there have been scratches> down the center of many of them.> We are now using adhesive in the float baths in spite of using the> charged slides. I would rather deal with background staining than tissue> falling off. Thermo has offered to replace bad slides, but who will replace> the time it takes to cut them not to mention the wasted tissues.> If I had known I would be going through this with the slides I would> not have bought the labeler.> > I wish you the best with this,> honestly,> Amos Brooks> > > Message: 5> Date: Tue, 10 Jun 2008 12:57:31 -0500> From: "Rosa Fields" &lt;rfields <@t> gidocs.net&gt;> Subject: RE: [Histonet] Slide printer> To: "Clark Stephen - Myrtle Beach"> &lt;Stephen.Clark1 <@t> hcahealthcare.com&gt;,> &lt;histonet <@t> lists.utsouthwestern.edu&gt;> Message-ID:> &lt;2F2611250DCD6549AA3D96CE8AF1F01801124072 <@t> giexchange.gidocs.net&gt;> Content-Type: text/plain; charset="iso-8859-1"> > We have just gotten a slide etcher from Thermo, manufactured by RA Lamb,> very simple to use, no ink!> Small footprint to boot (no pun intended!)> > > Rosa Fields, HT (ASCP)> Gastroenterology Specialties> Histology Supervisor> 4545 R Street> Lincoln, NE 68503> 402-465-4545> rfields <@t> gidocs.net> _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > __________________________________________________> Do You Yahoo!?> Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------> > Message: 7> Date: Thu, 12 Jun 2008 11:20:08 -0400> From: "Della Speranza, Vinnie" <dellav <@t> musc.edu>> Subject: RE: [Histonet] Logging in same type specimens consecutively> To: "'Barbara Rouse'" <kjnpeppa <@t> yahoo.com>, Histonet> <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <D2305C4BBC131346B131DD53870C62DFCB9A5E1525 <@t> EVS4.clinlan.local>> Content-Type: text/plain; charset="iso-8859-1"> > The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively.> > The reference is> Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002> > I am not aware that this is a checklist item in the accreditation requirements for AP> > Vinnie Della Speranza> Manager for Anatomic Pathology Services> 165 Ashley Avenue Suite 309> Charleston, South Carolina 29425> Tel: (843) 792-6353> Fax: (843) 792-8974> > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barbara Rouse> Sent: Wednesday, June 11, 2008 8:41 PM> To: Histonet> Subject: [Histonet] Logging in same type specimens consecutively> > Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ------------------------------> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > End of Histonet Digest, Vol 55, Issue 21> ****************************************
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