[Histonet] frozen sections

[Linda M Watson]

If they are not embedded in OCT then place them in 10% NBF-fix for 
appropriate amount of time prior to processing.You may have some freezer 
burn around the edges even after fixation but the rest of the tissue 
should be OK. If they are embedded in OCT you can either thaw it out 
remove the tissue as best as you can from the OCT and place the sample 
in 10% NBF(fix and process appropriately). If you want to section the 
frozen first and follow with formalin fixation prior to the IHC that 
sometimes works and gives better morphology than acetone fixation.

Good luck,
Linda

Helen E Johnson wrote:

>I have some frozen, unfixed tissues that I now wish I had fixed so that I
>could section them.  How difficult / possible would it be to cut and fix
>sections from these tissues that would be suitable for immunohistochemistry
>or fluorescence?
>Helen Johnson (hej01 <@t> health.state.ny.us)
>
>
>IMPORTANT NOTICE:  This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure.  It is intended only for the addressee.  If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments.  Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>