[Histonet] pAKT

[Valeria Berno]

Hi

sorry if I jump in the discussion but I am just starting to test the pAKT
from cell signalling (ser473) on brain cryosection.

could you please gave me some advise on the protocol you were using?

blocking with BSA,milk or serum?

did you use 1:25 dilution as they suggest?

triton 0.3% in blocking step and in primary antibody incubation??

some peculiar advise?

thanks

Valeria

Berno Valeria, PhD

EMBL- Mouse Biology Unit
Campus A.  Buzzati-Traverso
Via Ramarini,  32
00015, Monterotondo Scalo  (RM)
Italy

Tel:  +39 06 90091243
Fax: +39 06 90091406
email: valeria.berno <@t> embl.it
www.embl.it


<quote who="pruegg <@t> ihctech.net">
> cell signaling has a couple of alternative abs for pAKT they claim are
> better, i believe they are rab monoclonals, i have not tried them yet.
>
> Patsy
>
>
>> What Abs are people using for pAKT these days? The one I had working
>> so well in FFPE is no longer being made by Cell Signaling.
>>
>> Thanks,
>> Andrea
>> --
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Berno Valeria, PhD

EMBL- Mouse Biology Unit
Campus A.  Buzzati-Traverso
Via Ramarini,  32
00015, Monterotondo Scalo  (RM)
Italy

Tel:  +39 06 90091243
Fax: +39 06 90091406
email: valeria.berno <@t> embl.it
www.embl.it