[Histonet] IHC neg. controls (Friday rant)

[Patti Loykasek]

Hi Tim. I totally agree with you. I DO NOT think a negative should be run
for each antibody. In the comment of the ANP.2270 they even mention running
one for each antigen retrieval, but add if not then to use most aggressive
AR & they list the AR in order of aggresiveness. Running a negative for each
antibody is waste & if cited by a CAP inspector, I would appeal to CAP -
especially considering the way the comment is written. I was hoping someone
who is running a negative with each antibody could tell me their rationale
for doing this. 

Patti


> Patti, It does not say you need a separate negative tissue for each antibody,
> only "A" negative control tissue. One of each block, and for each protocol
> (ie, different pretreatments or detection system) would suffice.
> 
> 
> Tim Morken
> Technical Support Manager
> Lab Vision Products
> Anatomical Pathology
> ThermoFisher Scientific
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti Loykasek
> Sent: Friday, June 06, 2008 1:01 PM
> To: histonet
> Subject: [Histonet] IHC neg. controls (Friday rant)
> 
> Can someone rationalize to me the practice of running a negative control for
> every  antibody in an IHC workup? For example, six antibodies & six negative
> controls??? This makes me crazy. Just had a case, needle biopsy, where this
> occurred at an outside institution, and now we don¹t have enough tumor left to
> run more IHC & get a diagnosis. It borders on malpractice IMO.
> 
> On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The
> comment has the following (near the end):
> 
> A negative tissue control must be processed for each antibody in a given run.
> Any of the following can serve as a negative tissue control:
> 
> 1.             Multitissue blocks.  These can provide simultaneous positive
> and negative tissue controls, and are considered ³best practice²
> 2.             The positive control slide or patient test slides, if these
> slides contain tissue elements that should not react with the antibody.
> 3.             A separate negative tissue control slide.
> 
> I think it best to asses this negative tissue control on your positive
> controls that should contain negative elements. Plus, use known negative
> elements on the patient slides. The patient tissue is precious & these
> patients have undergone procedures that have associated morbidity. I can¹t see
> using up patient tissue for multiple negative controls & Having the patient
> have to undergo another procedure!
> Ok ­ I¹ll stop now.
> 
> Patti Loykasek
> 
> 
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intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
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