[Histonet] question

Tony Henwood AnthonyH <@t> chw.edu.au
Thu Jun 5 19:42:50 CDT 2008

I concur (now that's an old word)

Or, how about:

I am unanimous in this


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anthony
Sent: Friday, 6 June 2008 9:56 AM
To: Histonet <@t> lists.utsouthwestern.edu; Stephen Peters M.D.
Subject: Re: [Histonet] question

You have appeared to try every bluing agent possible, however in my
experience the most common cause of pale nuclei in frozen sections is
inadequate fixation.  What are you using?  The best I have used is 5%
Conc Formalin in 95% Ethanol for at least 30 sec and longer if time
All the best.
Tony Reilly

Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Ipswich Rd, 
Woolloongabba Q 4102
Ph: 07 32402412
Fax:07 32402930
tony_reilly <@t> health.qld.gov.au

>>> "Stephen Peters M.D." <petepath <@t> yahoo.com> 6/06/2008 1:34 am >>>
  You may also want to consider the thickness of your sections. A great
deal of our information is gatered at scanning magnification of 2x or
4x. If you are cutting at 3 or 
  4 microns or if your cryostat is offering a variety of thick and thin
the slides will 
  be pale at these powers compared to 5 or 6 micron sections. Presence
of a  lot of 
  nuclear "holes"is a sign it is cut very thin.   Also make sure your
hematoxalin is 
  changed regularly and is not growing "rock candy" in the bottom. Check
slide after it leaves the bluing and get to know the shade of blue that
represents a well stained slide. Remember when checking the slides, the
amount of blue will depend on the densityof nuclear material as well as
the thickness of the tissue. I find the actual shade 
  of blue tells me that it has been stained in hemotoxalin long enough. 

Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836

Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 

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