[Histonet] Pancreas RNA

Madeline.Branflick <@t> sanofi-aventis.com Madeline.Branflick <@t> sanofi-aventis.com
Thu Jun 5 14:12:10 CDT 2008

Does anyone have experience working with pancreas tissue for downstream
LCM?  Particularly FFPE and frozen collection procedures/protocols.  We
have tried both formats, and were unsuccessful in maintaining RNA
integrity.  We've tried to get around the endogenous ribonuclease issue
by snap freezing in chilled isopentane, or a direct fixation in 10% NBF.
Any help is greatly appreciated.

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