[Histonet] question

Victoria Baker bakevictoria <@t> gmail.com
Thu Jun 5 08:56:26 CDT 2008


For bluing reagents I've used several, ammonia water, saturated
lithium carbonate, 0.5% lithium carbonate and Scott's solution/water.
The Scott's solution was from Polyscientific in Bayshore NY and it's a
modified formula.  The original one I used, I think (I don't have it
in front of me) was from Sheehan.  What I found was most important
with the nuclear staining was that the hematoxylin had to as fresh as
possible and that the most critical steps were the removal of the OCT
media prior to staining as that seemed to give the sections that
greyish watery appearance.

Also, are you differentiating prior to bluing?  Just asking as you
didn't say what your protocol was.


On 6/5/08, Green, Kathy <kgreen <@t> hsh.org> wrote:
> Dear Fellow Histotechs,
> Our one pathologists'constantly complains that our frozen section slides
> are not blue enough.  We've tried leaving it in the Hematoxylin 2 longer
> & we even switched from ammonia water to Blue Buffer by Surgipath.  I'm
> asking what procedures and/or chemicals other labs use in their frozen
> section staining area.  Thanks for any input.
> Kathy Green, HT
> Manager Histology Laboratory
> Holy Spirit Hospital
> 503 N. 21st Street
> Camp Hill, PA  17011
> kgreen <@t> hsh.org
> (717) 763-2930
> Blackberry:  (717) 370-1726
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