[Histonet] xylene grades

[Merced Leiker]

Would the grade of xylenes used for deparaffinizing make a difference for 
immunostaining of certain (picky) epitopes?  We are using Certified A.C.S. 
xylenes.

What about rinsing in tap water vs. distilled water during rehydration?

The reason I'm asking is that we are having issues staining for c-kit (KIT, 
CD117) on FFPE tissue.   We've tried all different antigen retrievals, 
including no retrieval, and still aren't seeing any stain.

Any help would be greatly appreciated.  Thanks!


Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725