[Histonet] Agarose embedding

cmmathis1 <@t> bellsouth.net cmmathis1 <@t> bellsouth.net
Tue Jul 29 09:39:22 CDT 2008


I need to embed some thin tissue membranes in agarose.  Sigma lists 37 kinds.  Any suggestions as to which type to use.  Our protocol says to prepare a 2% solution and we tried this with an old bottle of agarose from Gibco that we found in the lab.  When I processed the block, it shrank a lot.  Any help would be greatly appreciated!
Cathy


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