[Histonet] Blue Zones

[Okland, Gloria A]

Hi All, 
 
We have been seeing a very random appearance of Blue Zones on our H&E
slides.  It affects one section, but not the next in serial sections and
if we restain the slides, they look better, and it seems to just be the
GI biopsies that we have trouble with.  It seems like the tissue is not
being stained by the eosin in these areas.  The slides are
deparraffinized and hydrated, and the hematoxylin stain looks ok, it is
just the Eosin.  Any suggestions that you may have, are greatly
appreciated, this have been an ongoing problem for some time now.
 
Thanks
Gloria
 
 

Gloria Okland HT (ASCP) 
Histology Supervisor 
Quest Diagnostics 
4770 Regent Blvd 
Irving, TX 75063 
972-536-8820 
972-916-3539 fax 

 


------------------------------------------
The contents of this message, together with any attachments, are
intended only for the use of the person(s) to which they are
addressed and may contain confidential and/or privileged
information. Further, any medical information herein is
confidential and protected by law. It is unlawful for unauthorized
persons to use, review, copy, disclose, or disseminate confidential
medical information. If you are not the intended recipient,
immediately advise the sender and delete this message and any
attachments. Any distribution, or copying of this message, or any
attachment, is prohibited.